Feasibility Study of the 3D Visualization at High Resolution of Intra-Cranial Rabbit Eyes With X-Ray CT Phase-Contrast Imaging
Feasibility Study of the 3D Visualization at High Resolution of Intra-Cranial Rabbit Eyes With X-Ray CT Phase-Contrast Imaging
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DOI：
10.1167/iovs.17-22273
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发表时间：
2017-11-01
影响因子：
4.4
通讯作者：
Tkacheva, Marina
中科院分区：
文献类型：
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作者：
Ivanishko, Yury;Bravin, Alberto;Tkacheva, Marina
目的.无论是在临床还是在临床前诊断研究中，都尚未实现以微米分辨率对眼球的整个体积进行颅内三维（3D）可视化。克服这一局限性可能会提供一个新的工具，临床和临床前研究的不同病理的各个部分的眼睛。本工作的目的是给第一个洞察力的体积可视化的高分辨率的整个摘除和颅内死后兔眼球。采用X线计算机断层相位衬度成像技术获得兔眼球摘除和颅内的三维模型。将图像与使用光学相干断层扫描（OCT）图像测量的图像进行比较。该实验是在欧洲同步辐射设施进行的。结合相位衬度成像、显微断层扫描和同步辐射特性提供的独特可能性，本文首次报道了全眼球的3D可视化，其各向同性体素尺寸为3.1 μ m（3）。由于X射线相衬成像相对于常规射线照相成像实现了上级性能，因此无需注射造影剂即可实现高图像对比度。在这项工作中开发的测量协议开辟了整个器官的体内高分辨率可视化的方式。
PURPOSE. The intracranial three-dimensional (3D) visualization of the whole volume of the eyeball at micrometric resolution has not been achieved yet either in clinical nor in preclinical diagnostic research. Overcoming this limitation may provide a new tool for clinical and preclinical studies of different pathologies of the various sections of the eye. The aim of this work is to give the first insight of a volumetric visualization at the high resolution of the entire enucleated and intracranial postmortem rabbit eyeballs.METHODS. X-ray computed tomography phase-contrast imaging was used to obtain 3D models of enucleated and intracranial rabbit eyes. Images were compared with the ones measured by using optical coherence tomography (OCT) images. The experiment was carried out at the European Synchrotron Radiation Facility.RESULTS. Combining the unique possibilities offered by phase-contrast imaging, microtomography, and the properties of synchrotron radiation, the 3D visualization of the whole eyeball, at an isotropic voxel size of 3.1 mu m(3), is reported here for the first time.CONCLUSIONS. High image contrast is achieved without the necessity of injection of contrast agents, thanks to the superior performances, achieved by x-ray phase-contrast imaging with respect to the conventional radiographic imaging. The measurement protocol developed within this work opens the way for in vivo high-resolution visualization of the entire organ.