Tumor lymphangiogenesis in inflammatory breast carcinoma: A histomorphometric study
Tumor lymphangiogenesis in inflammatory breast carcinoma: A histomorphometric study
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DOI：
10.1158/1078-0432.ccr-05-1142
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发表时间：
2005-11-01
影响因子：
11.5
通讯作者：
Vermeulen, PB
中科院分区：
文献类型：
--
作者：
Van der Auwera, I;Van den Eynden, GG;Vermeulen, PB
目的：在诊断时，几乎所有炎性乳腺癌（IBC）患者都发生了肿瘤细胞通过淋巴系统的转移性播散。本研究的目的是双重的：（a）以确定哪一个是最合适的标志物的淋巴管在原发性乳腺肿瘤和（B）比较组织形态计量淋巴管变量在IBC和non-IBC.Experimental设计：连续切片的10个IBC和10个non-IBC的免疫染色D240，LYVE-1，podoplanin，和PROM。采用D2-40/Ki-67双标法检测10例正常乳腺组织、29例IBC和56例非IBC的淋巴管相对面积、周长、计数和淋巴管内皮细胞增殖（LECP）。D2-40染色的瘤内淋巴管存在于80%的非IBC和82.8%的IBC中（P = 0.76）。在非IBC中，位于肿瘤实质中的淋巴管比位于肿瘤周围的淋巴管小且数量少（P < 0.0001），而在IBC中，瘤内和瘤周变量无显著差异。淋巴管所占的平均相对肿瘤面积在IBC中大于非IBC（P = 0.01）。IBC的LECP高于非IBC：IBC的LECP中位数为5.74%，非IBC的LECP中位数为1.83%（P = 0.005）。
Purpose: At the time of diagnosis, metastatic dissemination of tumor cells via the lymphatic system has occurred in nearly all patients with inflammatory breast cancer (IBC). The objective of this study was twofold: (a) to determine which is the most suitable marker of lymph vessels in primary breast tumors and (b) to compare histomorphometric lymph vessel variables in IBC and non-IBC.Experimental Design: Serial sections of 10 IBCs and 10 non-IBCs were immunostained for D240, LYVE-1, podoplanin, and PROM. Relative lymph vessel area, lymph vessel perimeters, and counts and lymphatic endothelial cell proliferation (LECP) were then measured in D2-40/Ki-67 double- immunostained sections of 10 normal breast tissues, 29 IBCs, and 56 non-IBCs.Results: D2-40 was the most suitable antibody for staining peritumoral and intratumoral lymph vessels. D2-40-stained intratumoral lymph vessels were present in 80% of non-IBCs and 82.8% of IBCs (P = 0.76). In non-IBC, lymph vessels located in the tumor parenchyma were smaller and less numerous than those at the tumor periphery (P < 0.0001) whereas in IBC, intratumoral and peritumoral variables were not significantly different. The mean relative tumor area occupied by lymph vessels was larger in IBC than in non-IBC (P = 0.01). LECP at the tumor periphery was higher in IBC than in non-IBC: median LECP was 5.74% in IBC versus 1.83% in non-IBC (P = 0.005).Conclusions: The high LECP in IBC suggests that lymphangiogenesis contributes to the extensive lymphatic spread of IBC.