EVIDENCE THAT THE ROUS-SARCOMA VIRUS TRANSFORMING GENE-PRODUCT PHOSPHORYLATES PHOSPHATIDYLINOSITOL AND DIACYLGLYCEROL
EVIDENCE THAT THE ROUS-SARCOMA VIRUS TRANSFORMING GENE-PRODUCT PHOSPHORYLATES PHOSPHATIDYLINOSITOL AND DIACYLGLYCEROL
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DOI：
10.1073/pnas.81.7.2117
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
ERIKSON, RL
中科院分区：
文献类型：
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作者：
SUGIMOTO, Y;WHITMAN, M;ERIKSON, RL
研究了纯化的劳斯肉瘤病毒转化基因产物pp 60 v-src磷酸化磷脂酰肌醇和甘油二酯的能力。磷脂酰肌醇被磷酸化形成单磷酸化衍生物和二磷酸化衍生物。将1，2-二酰甘油磷酸化形成磷脂酸。这些活性显示出与pp 60 v-SRC的酪蛋白激酶活性相同的热不稳定性和对抑制剂相同的敏感性。当血清饥饿的鸡胚成纤维细胞转化的病毒突变体的温度敏感的转化从nonpermissive到允许的温度，增加了50-100%的标记磷脂酰肌醇4-磷酸，磷脂酰肌醇4，5-二磷酸，和磷脂酸被观察到，与未感染的细胞相比。
The ability of purified Rous saroma virus transforming gene product, pp60v-src, to phosphorylate phosphatidylinositol and diacylglycerol was investigated. Phosphatidylinositol was phosphorylated to form both mono- and diphosphorylated derivatives. 1,2-Diacylglycerol was phosphorylated to form phosphatidic acid. These activities showed the same thermolability and the same sensitivity to inhibitors as shown by the casein kinsae activity of pp60v-src. When serum-starved chicken embryo fibroblasts transformed by a virus mutant temperature-sensitive for transformation were shifted from the nonpermissive to permissive temperature, an increase of 50-100% in the labeling of phosphatidylinositol 4-phosphate, phosphatidylinositol 4,5-bisphosphate, and phosphatidic acid was observed, as compared to uninfected cells.