Proteases, actinidin, papain and trypsin reduce oral biofilm on the tongue in elderly subjects and in vitro
Proteases, actinidin, papain and trypsin reduce oral biofilm on the tongue in elderly subjects and in vitro
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DOI：
10.1016/j.archoralbio.2017.04.035
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发表时间：
2017-10-01
影响因子：
3
通讯作者：
Komasa, Yutaka
中科院分区：
文献类型：
--
作者：
Mugita, Naho;Nambu, Takayuki;Komasa, Yutaka
目的：牙菌斑是老年人口腔疾病的致病因素，也是呼吸道感染的潜在宿主。因此，迫切需要开发有效的方法来去除口腔生物膜。本研究的目的是探讨蛋白酶对口腔生物膜的形成和removal.Design：猕猴桃蛋白酶，半胱氨酸蛋白酶，对舌苔的去除在体内的影响进行了评估后，口服蛋白酶片。采用微量滴定板法研究了胰蛋白酶、木瓜蛋白酶和猕猴桃蛋白酶对单种放线菌生物膜和多种放线菌生物膜的影响。抗菌试验和有限的菌毛轴蛋白的蛋白水解也进行了澄清口腔生物膜remove.Results的潜在机制：片剂含有猕猴桃去除老年人的舌苔。蛋白酶木瓜蛋白酶、猕猴桃蛋白酶和胰蛋白酶显著减少口腔放线菌生物膜。木瓜蛋白酶和胰蛋白酶能有效地消化放线菌的主要菌毛蛋白FimP和FimA。猕猴桃蛋白酶、木瓜蛋白酶和胰蛋白酶可降低体外重建的多物种生物膜。木瓜蛋白酶和胰蛋白酶抑制多物种生物膜的形成在vitro.Conclusions：这项研究表明，蛋白酶减少口腔生物膜在体内老年人和体外，并表明，蛋白酶抑制菌毛和抑制生物膜的形成。
Objective: Dental plaque is a causative factor for oral disease and a potential reservoir for respiratory infection in the elderly. Therefore, there is a critical need for the development of effective methods to remove oral biofilm. The objective of this study was to investigate the effect of proteases on oral biofilm formation and removal.Design: The in vivo effect of actinidin, a cysteine protease, on the removal of tongue coating was assessed after orally taking a protease tablet. Effects of the proteases trypsin, papain and actinidin on Actinomyces mono species biofilm and multispecies biofilm that was reconstructed using a plaque sample from the tongue coating were investigated using the microliter plate method. Antimicrobial tests and limited proteolysis of fimbrial shaft proteins were also performed to clarify underlying mechanisms of oral biofilm removal.Results: Tablets containing actinidin removed tongue coating in elderly subjects. Oral Actinomyces biofilm was significantly reduced by the proteases papain, actinidin and trypsin. Papain and trypsin effectively digested the major fimbrial proteins, FimP and FimA, from Actinomyces. Actinidin, papain and trypsin reduced multispecies biofilm that was reconstructed in vitro. Papain and trypsin inhibited formation of multispecies biofilm in vitro.Conclusions: This study shows that proteases reduced oral biofilm in vivo in elderly subjects and in vitro, and suggests that protease digests fimbriae and inhibits biofilm formation.