Rapid Detection of a Cocaine-Binding Aptamer Using Biological Nanopores on a Chip
Rapid Detection of a Cocaine-Binding Aptamer Using Biological Nanopores on a Chip
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DOI：
10.1021/ja2026085
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发表时间：
2011-06-08
影响因子：
15
通讯作者：
Takeuchi, Shoji
中科院分区：
文献类型：
--
作者：
Kawano, Ryuji;Osaki, Toshihisa;Takeuchi, Shoji
本文介绍了一种利用膜蛋白通道结合DNA适体快速、高选择性检测可卡因的方法。DNA适体以高选择性识别可卡因分子。我们成功地检测到低浓度的可卡因（300 ng/mL，药物测试截止限）在60秒内使用嵌入在微芯片中的生物纳米孔。
This paper describes a methodology for the rapid and highly selective detection of cocaine using a membrane protein channel combined with a DNA aptamer. The DNA aptamer recognizes the cocaine molecule with high selectivity. We successfully detected a low concentration of cocaine (300 ng/mL, the drug test cutoff limit) within 60 s using a biological nanopore embedded in a microchip.