Real-time quaking-induced conversion
Real-time quaking-induced conversion
复制标题
实时地震引起的转换
作者：
Ryuichiro Atarashi;K. Sano;K. Satoh;N. Nishida
我们最近开发了一种新的体外扩增技术，命名为“实时quaking-induced转换（RT-QUIC）”，用于检测异常形式的朊病毒蛋白（PrPSc）在容易获得的标本，如脑脊液（CSF）。在对来自日本和澳大利亚患者的200多份CSF样本进行评估后，我们没有发现假阳性的情况，并且正确诊断散发性克雅氏病（sCJD）的准确率超过80%。此外，RT-QUIC可应用于其他朊病毒疾病，包括羊瘙痒病、慢性消耗性疾病（CWD）和牛海绵状脑病（BSE），并且当与样品的终点稀释组合时能够定量朊病毒接种活性。这些结果表明，RT-QUIC，其高灵敏度和特异性，将是一个很大的使用作为一个早期，快速和特异性检测朊病毒疾病。
We recently developed a new in vitro amplification technology, designated “real-time quaking-induced conversion (RT-QUIC)”, for detection of the abnormal form of prion protein (PrPSc) in easily accessible specimens such as cerebrospinal fluid (CSF). After assessment of more than 200 CSF specimens from Japanese and Australian patients, we found no instance of a false positive, and more than 80% accuracy for the correct diagnosis of sporadic Creutzfeldt–Jakob disease (sCJD). Furthermore, the RT-QUIC can be applied to other prion diseases, including scrapie, chronic wasting disease (CWD), and bovine spongiform encephalopathy (BSE), and is able to quantify prion seeding activity when combined with an end-point dilution of samples. These results indicate that the RT-QUIC, with its high sensitivity and specificity, will be of great use as an early, rapid and specific assay for prion diseases.
影响因子：
158.5
作者：
Hsich, G;Kinney, K;Harrington, MG
通讯作者：
Harrington, MG