Deoxyribonucleic acid replication and germ cell apoptosis during spermatogenesis in the rabbit.
Deoxyribonucleic acid replication and germ cell apoptosis during spermatogenesis in the rabbit.
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DOI：
10.1002/j.1939-4640.2002.tb02613.x
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发表时间：
2002-03
影响因子：
--
通讯作者：
J. Blanco‐Rodríguez
中科院分区：
文献类型：
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作者：
J. Blanco‐Rodríguez
更深入地了解精子发生过程中某些事件的发生，如DNA复制或生殖细胞凋亡，对于提高我们对生精上皮生物学的理解具有重要意义。然而，对这些事件的研究仅限于小型啮齿动物。本文用溴脱氧尿嘧啶核苷标记分析了DNA合成的发生，并用TUNEL法分析了生殖细胞的凋亡率。与以前对这种动物精原细胞增殖的描述相反，我们的结果表明DNA在8/1、2、4、5、6和7阶段复制，这相当于大鼠和小鼠的DNA合成阶段。精原细胞和精母细胞的凋亡一般发生在第2期和第4期，此时晚期精母细胞结束粗线期或进行减数分裂，这一结果与以前在大鼠睾丸中观察到的结果一致。
A deeper understanding of the occurrence of some events, such as DNA replication or germ cell apoptosis during spermatogenesis, is important for improving our understanding of seminiferous epithelium biology. However, studies on these events have been restricted to small rodents. This paper analyzes the occurrence of DNA synthesis by bromo-deoxy-uridine labeling, as well as the incidence of germ cell apoptosis by the TUNEL assay in the rabbit. In contrast to previous descriptions of spermatogonium proliferation in this animal, our results indicate that DNA replicates at stages 8/1, 2, 4, 5, 6, and 7, which are equivalent to the stages at which DNA synthesis occurs in rats and mice. Apoptosis of both spermatogonia and spermatocytes generally occurred at stages 2 and 4, when late spermatocytes end the pachytene stage or undergo meiotic divisions, and these results are consistent with previous observations in the rat testis.