Imaging mass spectrometry at cellular length scales
Imaging mass spectrometry at cellular length scales
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DOI：
10.1038/nprot.2007.117
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发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Heeren, Ron M. A.
中科院分区：
文献类型：
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作者：
Altelaar, A. F. Maarten;Luxembourg, Stefan L.;Heeren, Ron M. A.
成像质谱仪(IMS)可以直接在组织切片、单个细胞和许多其他生物表面上直接研究分子含量的身份和空间分布。在该方案中，我们介绍了使用基质增强(ME)和金属辅助(META)二次离子质谱仪(SIMS)以及基质辅助激光解吸/电离(MALDI)IMS从组织切片中检索分子信息所需的步骤。这些技术需要特定的样品制备步骤，以最大限度地减少样品分析物的再分配或修改，达到最佳信号强度。经过仔细的样品准备，不同的IMS方法提供了一种独特的发现工具，例如，在(I)药物传输和摄取、(Ii)生物处理步骤和(Iii)生物标志物分布的调查中。为了从IMS产生的海量数据集中提取相关信息，人们开发了新的生物信息学方法。方案的持续时间在很大程度上取决于样本大小和使用的技术，但平均需要大约5小时。
Imaging mass spectrometry ( IMS) allows the direct investigation of both the identity and the spatial distribution of the molecular content directly in tissue sections, single cells and many other biological surfaces. In this protocol, we present the steps required to retrieve the molecular information from tissue sections using matrix-enhanced ( ME) and metal-assisted ( MetA) secondary ion mass spectrometry ( SIMS) as well as matrix-assisted laser desorption/ionization ( MALDI) IMS. These techniques require specific sample preparation steps directed at optimal signal intensity with minimal redistribution or modification of the sample analytes. After careful sample preparation, different IMS methods offer a unique discovery tool in, for example, the investigation of ( i) drug transport and uptake, ( ii) biological processing steps and ( iii) biomarker distributions. To extract the relevant information from the huge datasets produced by IMS, new bioinformatics approaches have been developed. The duration of the protocol is highly dependent on sample size and technique used, but on average takes approximately 5 h.