Selenium supplementation through Se-rich dietary matrices can upregulate the anti-inflammatory responses in lipopolysaccharide-stimulated murine macrophages
Selenium supplementation through Se-rich dietary matrices can upregulate the anti-inflammatory responses in lipopolysaccharide-stimulated murine macrophages
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DOI：
10.1080/09540105.2017.1343805
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发表时间：
2017-01-01
影响因子：
3
通讯作者：
Prakash, N. Tejo
中科院分区：
文献类型：
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作者：
Dhanjal, Noorpreet Inder Kaur;Sharma, Siddharth;Prakash, N. Tejo
从自然富集的来源，如谷类作物硒的可获得性可能被用作硒补充剂，以支持营养需求。膳食硒补充剂，作为富硒小麦提取物，对RAW 264.7巨噬细胞通过增加细胞硒蛋白谷胱甘肽过氧化物酶1（GPx-1）的表达，在存在或不存在的脂多糖（LPS）处理增强抗氧化能力。以亚硒酸钠（SS）、含硒小麦提取物（SeW）和含硒小麦提取物与rMET酶处理（SeW + rMET）的形式以三种不同浓度补充硒。与SeW处理的细胞相比，补充有SS和SeW + rMET的细胞显示GPx-1表达增加。此外，SeW + rMET下调LPS诱导的环氧合酶-2、微粒体PGE合酶-1和诱导型一氧化氮合酶的表达。硒缺乏的细胞，而造血PGD合成酶的表达上调。这表明SeSup有效地调节炎症反应的表达，表明膳食硒补充剂的潜在益处。
The accessibility of selenium from naturally enriched sources such as cereals crops can potentially be used as selenium supplements to support nutritional requirements. Dietary selenium supplementation, as Se-rich wheat extracts, on RAW264.7 macrophage cells enhanced the antioxidant capacity via augmentation of cellular selenoprotein glutathione peroxidase 1 (GPx-1) expression in the absence or presence of lipopolysaccharide (LPS) treatment. Cells were supplemented with Se in the form of sodium selenite (SS), seleniferous wheat extract (SeW) and seleniferous wheat extract with rMETase treatment (SeW + rMET) at three different concentrations. Cells supplemented with SS and SeW + rMET showed increase in GPx-1 expression as compared to SeW-treated cells. SeW + rMET, further, downregulated the LPS-induced expression of cyclooxygenase-2, microsomal PGE synthase-1 and inducible nitric oxide synthase w.r.t. Se-deficient cells, while the expression of haematopoietic PGD synthase was upregulated. This demonstrates SeSup effectively modulates the expression of inflammatory responses, indicating the potential benefits of dietary selenium supplementation.