Actinomadura harenae sp. nov., a novel actinomycete isolated from the sea sand in Sanya
Actinomadura harenae sp. nov., a novel actinomycete isolated from the sea sand in Sanya
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马杜拉放线菌属
DOI：
10.1099/ijsem.0.003819
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发表时间：
--
影响因子：
2.8
通讯作者：
Wensheng Xiang
中科院分区：
文献类型：
--
作者：
Jiangmeihui Hu;Chuanyu Han;Bing Yu;Junwei Zhao;Xiaowei Guo;Yue Shen;Xiangjing Wang;Wensheng Xiang
A novel actinomycete, designated strain NEAU-Ht49T, was isolated from sea sand sampled in Sanya and characterized by using a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain NEAU-Ht49Twas most closely related toActinomadura rhizosphaeraeSDA37T(98.8 %),Actinomadura logoneensisNEAU-G17T(98.6 %),Actinomadura oligosporaATCC 43269T(98.6 %) andActinomadura gamaensisNEAU-Gz5T(98.6 %). The results of phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain NEAU-Ht49Tformed a cluster withA. rhizosphaeraeSDA37T,A. logoneensisNEAU-G17T,A. oligosporaATCC 43269T,A. gamaensisNEAU-Gz5TandActinomadura rupiterraeCS5-AC15T(96.4 %). Meso-diaminopimelic acid was detected in its cell walls and glucose, madurose, mannose and ribose were detected in whole-cell hydrolysate. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositolmannoside and two unidentified lipids. The majoy menaquinone was MK-10(H6) and the minor menaquinones were MK-9(H4) and MK-9(H8). The major fatty acids were C16 : 0, C18 : 1ω9c, 10-methyl C18 : 0and iso-C16 : 0. Moreover, morphological and chemotaxonomic characteristics of properties of strain NEAU-Ht49Talso confirmed the affiliation of the isolate to the genusActinomadura. However, DNA–DNA relatedness, physiological and biochemical data showed that strain NEAU-Ht49Tcould be distinguished from its closest relatives. Therefore, strain NEAU-Ht49Trepresents a novel species of the genusActinomadura, for which the nameActinomadura harenaesp. nov. is proposed, with strain NEAU-Ht49T(=CGMCC 4.7499T=JCM 32659T) as the type strain.
