Optimization of a minimal sample preparation protocol for imaging mass spectrometry of unsectioned juvenile invertebrates.
Optimization of a minimal sample preparation protocol for imaging mass spectrometry of unsectioned juvenile invertebrates.
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用于未切片幼年无脊椎动物成像质谱分析的最小样品制备方案的优化。
DOI：
10.1002/jms.4458
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Sanchez,LauraM
中科院分区：
文献类型：
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作者：
Zink,KatherineE;Tarnowski,DeniseA;Mandel,MarkJ;Sanchez,LauraM
组织切片长期以来一直是成像质谱法应用的主题，但最近该技术已适用于许多其他目的，包括细菌菌落和3D细胞培养。在这里，我们提出了一个简单的制备方法，未切片的无脊椎动物组织，而不需要固定，包埋，或切片。该协议被用来成功地准备夏威夷短尾乌贼幼体进行分析，并得到的数据检测离子，对应的化合物存在于主机中，只有在其共生殖民byVibrio fischeri。
Tissue sections have long been the subject matter for the application of imaging mass spectrometry, but recently the technique has been adapted for many other purposes including bacterial colonies and 3D cell culture. Here, we present a simple preparation method for unsectioned invertebrate tissue without the need for fixing, embedding, or slicing. The protocol was used to successfully prepare a Hawaiian bobtail squid hatchling for analysis, and the resulting data detected ions that correspond to compounds present in the host only during its symbiotic colonization byVibrio fischeri.