Quercetin regulates inflammation, oxidative stress, apoptosis, and mitochondrial structure and function in H9C2 cells by promoting PVT1 expression
Quercetin regulates inflammation, oxidative stress, apoptosis, and mitochondrial structure and function in H9C2 cells by promoting PVT1 expression
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槲皮素通过促进 PVT1 表达来调节 H9C2 细胞中的炎症、氧化应激、细胞凋亡以及线粒体结构和功能。
DOI：
10.1016/j.acthis.2021.151819
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发表时间：
2021-12-01
影响因子：
2.5
通讯作者：
Yan, Xisheng
中科院分区：
文献类型：
--
作者：
Li, Fen;Liu, Jianguang;Yan, Xisheng
目的：为探讨槲皮素（quercetin）对H9 C2细胞炎症、氧化应激、凋亡及线粒体结构与功能的影响及其可能机制，材料和方法：H9 C2细胞来源于中国科学院上海生命科学研究院，随机分为6组：对照组、模型组、PVT 1过表达组（OV）、槲皮素组、OV +槲皮素组和NAC组。进行CCK-8测定以检查细胞增殖。流式细胞术检测细胞凋亡、细胞膜电位和ROS水平。采用酶联免疫吸附试验（ELISA）和生化试剂盒检测内皮型一氧化氮合酶（eNOS）、丙二醛（MDA）和超氧化物歧化酶（SOD）的表达。Western blotting法测定p-DRP 1（s637）、MFN 2、NF-κ B、p-NF-κ B、IkB和p-IkB的水平。通过RT-PCR检测IL-6、IL-10、TNF-α和IL-1 β mRNA表达。结果：模型组MDA、p-NF-kappa B、p-IKB、IL-6、IL-1 β、TNF-α表达水平及细胞凋亡率均显著高于对照组（P < 0.05）。模型组细胞增殖率、IL-10、SOD、eNOS、ATP水平均明显低于模型组（P < 0.05）。此外，与模型组相比，奥曲肽、槲皮素、槲皮素+奥曲肽和NAC组的MDA表达明显降低（P < 0.05），而SOD、eNOS和ATP水平则升高。结论：槲皮素促进H9 C2细胞增殖，同时抑制炎症、氧化应激和细胞凋亡，减轻线粒体结构和功能的紊乱。这些作用是通过促进PVT 1表达来实现的。
Objective: To investigate the effect and potential mechanism of quercetin on inflammation, oxidative stress, apoptosis, and mitochondrial structure and function in H9C2 cells.Materials and methods: H9C2 cells were obtained from the Shanghai Institutes for Biological Sciences, Chinese Academy of Science, and randomly divided into six groups: control, model, PVT1 overexpression (OV), quercetin, OV + quercetin, and NAC groups. The CCK-8 assay was performed to examine cell proliferation. Flow cytometry was used to examine cell apoptosis, cell membrane potential, and ROS levels. The expression of endothelial nitric oxide synthase (eNOS), malondialdehyde (MDA), and superoxide dismutase (SOD) was measured by ELISA and a Biochemical kit. Western blotting was used to determine the levels of p-DRP1 (s637), MFN2, NF-kB, p-NF-kB, IkB, and p-IkB. IL-6, IL-10, TNF-alpha, and IL-1 beta mRNA expression was examined by RT-PCR. Electron microscopy was used to observe the structure of mitochondria in H9C2 cells.Results: MDA, p-NF-kappa B, p-IKB, IL-6, IL-1 beta, and TNF-alpha expression levels, and the cell apoptosis rate were signif-icantly higher in the model group than in the control group (P < 0.05). In contrast, the cell proliferation rate and IL-10, SOD, eNOS, and ATP levels were significantly lower in the model group (P < 0.05). Moreover, MDA expression was significantly lower in the OV, quercetin, quercetin + OV, and NAC groups than in the model group (P < 0.05), while SOD, eNOS, and ATP levels were higher. The electron microscopy results showed that PVT1 overexpression or quercetin treatment could inhibit inflammation-induced mitochondrial fission and promote mitochondrial fusion.Conclusion: Quercetin promotes the proliferation of H9C2 cells, while inhibiting inflammation, oxidative stress, and cell apoptosis, and alleviating the structural and functional dysfunction of mitochondria. These effects are achieved by promoting PVT1 expression.