Separation and identification of ceramides derived from human plasma sphingomyelins.
Separation and identification of ceramides derived from human plasma sphingomyelins.
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人血浆鞘磷脂中神经酰胺的分离和鉴定。
DOI：
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发表时间：
1969
影响因子：
6.5
通讯作者：
L. Samuelsson
中科院分区：
文献类型：
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作者：
B. Samuelsson;L. Samuelsson
人血浆中的鞘磷脂经磷脂酶C酶解转化为神经酰胺。乙酰化后的神经酰胺通过含有硝酸银的硅胶上的薄层色谱法分级分离。通过这种方法获得的四个主要馏分随后转化为二-O-三甲基甲硅烷基醚衍生物，并通过气液色谱法在1%OV-1上分离。2-11组分可以在四个级分中的每一个中区分。通过质谱分析从气相色谱仪出现的主要馏分，并鉴定其主要分子种类。两个气相色谱级分含有基本上纯的分子物质，即N-二十四烯酰基鞘氨醇和N-二十四烯酰基鞘氨醇-4，14-二烯碱。
Sphingomyelins from human blood plasma have been converted into ceramides by enzymatic hydrolysis with phospholipase C. After acetylation the ceramides were fractionated by thin-layer chromatography on silica gel containing silver nitrate. Four main fractions obtained by this method were subsequently converted to di-O-trimethylsilyl ether derivatives and separated by gas-liquid chromatography on 1% OV-1. 2-11 components could be distinguished in each of the four fractions. The major fractions emerging from the gas chromatograph were analyzed by mass spectrometry and their main molecular species were identified. Two of the gas chromatographic fractions contained essentially pure molecular species, namely N-tetracosenoyl sphingosine and N-tetracosenoylsphinga-4, 14-dienine.