Histological assessment of the early enthesitis lesion in spondyloarthropathy
Histological assessment of the early enthesitis lesion in spondyloarthropathy
复制标题
DOI：
10.1136/ard.61.6.534
复制
发表时间：
2002-06-01
影响因子：
27.4
通讯作者：
Emery, P
中科院分区：
文献类型：
--
作者：
McGonagle, D;Marzo-Ortega, H;Emery, P
目的：探讨早期脊柱关节病(SpA)急性终末期病变的组织学改变。方法：4例足底筋膜炎和1例髌腱末端炎经MRI和B超确诊为急性终末期病变。用Jamshedi穿刺针进行超声引导下穿刺点活检，取2例脊柱移植手术患者的对照组织，采用免疫组织化学和免疫组织化学方法，结合CD3、CD8、CD34和CD68标记，采用免疫组织化学和免疫组织化学方法检测穿刺点的细胞浸润情况。结果：SpA组插入点结构异常，血管增多，细胞浸润增多。纤维软骨末端以巨噬细胞为主，附着点淋巴细胞稀少。结论：这些初步发现有助于更好地了解早期SpA的病理机制。
Objectives: To describe the histological changes in acute enthesopathy in early spondyloarthropathies (SpA).Methods: Clinically evident acute enthesopathy was confirmed by magnetic resonance imaging and ultrasonography in four cases of plantar fasciitis and one case of patellar tendon enthesitis. Ultrasound guided biopsy of insertional points was carried out with a Jamshedi needle, Control tissue was obtained from two subjects undergoing spinal grafting surgery, Standard histochemistry and immunohistochemistry analysis using the avidin-biotin immunoperoxidase complex method employing markers against CD3, CD8, CD34, and CD68 was used to determine cellular infiltrates at the insertion point.Results: The enthesis architecture was abnormal in the SpA group, with increased vascularity and cellular infiltration compared with normal subjects. The predominant infiltrating cell at the enthesis fibrocartilage was the macrophage, but there was a paucity of lymphocytes at the insertion point.Conclusion: These preliminary findings have implications for a better understanding of the pathology in early SpA.