Systematic analysis, by the yeast two-hybrid, of protein interaction between components of the type II secretory machinery of Erwinia chrysanthemi
Systematic analysis, by the yeast two-hybrid, of protein interaction between components of the type II secretory machinery of Erwinia chrysanthemi
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DOI：
10.1016/j.resmic.2003.10.001
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发表时间：
2004-03-01
影响因子：
2.6
通讯作者：
Py, B
中科院分区：
文献类型：
--
作者：
Douet, V;Loiseau, L;Py, B
II型系统允许在几种革兰氏阴性病原体中分泌多种酶和毒素。在欧文氏菌中，14个Out蛋白是构建II型器官所必需的。我们进行了系统的双杂交分析，以测试外蛋白的周质区域之间的相互作用。使用这种方法获得的结果表明，OutJ（一个假的pseudopilin）能够相互作用G）OutD，外膜分泌素，（ii）Outl，主要位于周质，和（iii）OutL，内膜蛋白。总之，这些结果表明，OutJ参与了多个伙伴关系。这些伙伴关系的整体架构的II型分泌机制的影响进行了讨论。(C)2003年，Elsevier SAS。All rights reserved.
Type II systems allow for the secretion of numerous enzymes and toxins in several Gram-negative pathogens. In Erwinia chrysanthemi, 14 Out proteins are necessary for building the type II apparatus. We performed a systematic two-hybrid analysis to test interactions between the periplasmic regions of the Out proteins. Results obtained using this approach suggested that OutJ (a pseudopilin) was able to interact with G) OutD, the outer membrane secretin, (ii) Outl, mainly located in the periplasm, and (iii) OutL, an inner membrane protein. Taken together, these results suggest that OutJ is involved in multiple partnerships. Implications of these partnerships in the overall architecture of the type II secretion machinery are discussed. (C) 2003 Elsevier SAS. All rights reserved.