The human eye expresses high levels of CB1 cannabinoid receptor mRNA and protein
The human eye expresses high levels of CB1 cannabinoid receptor mRNA and protein
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DOI：
10.1046/j.1460-9568.2000.01027.x
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发表时间：
2000-03-01
影响因子：
3.4
通讯作者：
Pani, L
中科院分区：
文献类型：
--
作者：
Porcella, A;Maxia, C;Pani, L
我们使用逆转录酶聚合酶链反应来检测中央和外周大麻素受体（分别为CB 1和CB 2）mRNA的表达，并使用蛋白质印迹法来显示CB 1蛋白在人眼亚区域中的存在。与正常化参考基因β 2微球蛋白相比，CB 2 mRNA转录物检测不到，而CB 1 mRNA水平在人视网膜（25.8 +/- 2.46%）、睫状体（210 +/- 11.55%）和虹膜（62.7 +/- 5.94%）中显著表达。CB 1基因编码一种功能性蛋白质，可通过针对CB 1受体氨基末端（残基1-77）产生的特异性纯化抗体在相同区域检测到糖基化（63 kDa）和非糖基化（54 kDa）形式。这些结果进一步支持了CB 1受体在控制眼内压中的作用，有助于解释大麻的抗青光眼特性。
We used reverse transcriptase polymerase chain reaction to detect the expression of the central and peripheral cannabinoid receptors (CB1 and CB2, respectively) mRNA, and Western blotting to show the presence of the CB1 protein in subregions of the human eye. CB2 mRNA transcripts were undetectable, while levels of CB1 mRNA were significantly expressed in the human retina (25.8 +/- 2.46%), ciliary body (210 +/- 11.55%) and iris (62.7 +/- 5.94%) when compared with those of the normalizing reference gene beta(2) microglobulin. The CB1 gene encodes a functional protein which is detected in its glycosylated (63 kDa) and unglycosylated (54 kDa) form in the same areas by a specific purified antibody raised against the amino terminus (residues 1-77) of the CB1 receptor. These results further support the proposed role of the CB1 receptor in controlling intraocular pressure, helping to explain the antiglaucoma properties of marijuana.