Raman microspectroscopy:: A comparison of point, line, and wide-field imaging methodologies
Raman microspectroscopy:: A comparison of point, line, and wide-field imaging methodologies
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DOI：
10.1021/ac034169h
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发表时间：
2003-08-15
影响因子：
7.4
通讯作者：
Levin, IW
中科院分区：
文献类型：
--
作者：
Schlücker, S;Schaeberle, MD;Levin, IW
三种不同的拉曼显微光谱成像方法使用一个单一的实验配置进行比较，即，点和线映射，作为串行成像方法的代表，和直接或宽场拉曼成像采用液晶可调谐滤波器进行调查。拉曼成像数据采集与等效的低功率514.5 nm激光激发和冷却CCD相机进行了分析，相对于采集时间，图像质量，空间分辨率，强度分布沿着空间坐标，和光谱信噪比（SNR）。点和线映射技术提供了类似的SNR和重建的拉曼图像的空间分辨率为-1.1 μ m。相比之下，更高的空间分辨率是通过直接，全球成像（类似于313纳米），允许微妙的形态特征的测试样品，以解决。
Three different Raman microspectroscopic imaging methodologies using a single experimental configuration are compared; namely, point and line mapping, as representatives of serial imaging approaches, and direct or wide-field Raman imaging employing liquid-crystalline tunable filters are surveyed. Raman imaging data acquired with equivalent low-power 514.5-nm laser excitation and a cooled CCD camera are analyzed with respect to acquisition times, image quality, spatial resolution, intensity profiles along spatial coordinates, and spectral signal-to-noise ratios (SNRs). Point and line mapping techniques provide similar SNRs and reconstructed Raman images at spatial resolutions of -1.1 mum. In contrast, higher spatial resolution is obtained by direct, global imaging (similar to313 nm), allowing subtle morphological features on test samples to be resolved.