A family of dynein genes in Drosophila melanogaster.
A family of dynein genes in Drosophila melanogaster.
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DOI：
10.1091/mbc.5.1.45
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发表时间：
1994
影响因子：
3.3
通讯作者：
K. Rasmusson;M. Serr;J. Gepner;I. Gibbons;Thomas S. Hays
中科院分区：
文献类型：
--
作者：
K. Rasmusson;M. Serr;J. Gepner;I. Gibbons;Thomas S. Hays
我们报告了7个果蝇动力蛋白重链基因的鉴定和初步表征。每个基因都是单拷贝的，并映射到一个独特的基因组位置。部分克隆的序列分析表明，每个编码的推定动力蛋白水解ATP结合位点的动力蛋白，包括一个共识磷酸结合（P-环）基序的高度保守的部分。其中一个克隆来源于果蝇细胞质动力蛋白重链基因Dhc 64 C，该基因与其他生物体的细胞质动力蛋白同种型具有广泛的氨基酸同一性。另外两个果蝇动力蛋白克隆在氨基酸水平上与海胆轴丝动力蛋白的β重链的相应区域具有85%和90%的同一性。所有7个动力蛋白相关序列的探针与适当大小的转录物（约14个酶）杂交，以编码特征性高分子量动力蛋白重链多肽。Dhc 64 C转录本在来自卵巢、胚胎和睾丸的RNA中容易检测到。其余六个基因中的五个基因的转录本在睾丸以外的组织中也以少得多的量被检测到。除了一个动力蛋白转录本在睾丸中的表达水平相当，这表明它们参与鞭毛轴丝组装和运动。
We report the identification and initial characterization of seven Drosophila dynein heavy chain genes. Each gene is single copy and maps to a unique genomic location. Sequence analysis of partial clones reveals that each encodes a highly conserved portion of the putative dynein hydrolytic ATP-binding site in dyneins that includes a consensus phosphate-binding (P-loop) motif. One of the clones is derived from a Drosophila cytoplasmic dynein heavy chain gene, Dhc64C, that shows extensive amino acid identity to cytoplasmic dynein isoforms from other organisms. Two other Drosophila dynein clones are 85 and 90% identical at the amino acid level to the corresponding region of the beta heavy chain of sea urchin axonemal dynein. Probes for all seven of the dynein-related sequences hybridize to transcripts that are of the appropriate size, approximately 14 kilobases, to encode the characteristic high molecular weight dynein heavy chain polypeptides. The Dhc64C transcript is readily detected in RNA from ovaries, embryos, and testes. Transcripts from five of the six remaining genes are also detected in much lesser amounts in tissues other than testes. All but one of the dynein transcripts are expressed at comparable levels in testes suggesting their participation in flagellar axoneme assembly and motility.