Recent advances in intracellular and in vivo ROS sensing: focus on nanoparticle and nanotube applications.
Recent advances in intracellular and in vivo ROS sensing: focus on nanoparticle and nanotube applications.
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DOI：
10.3390/ijms130910660
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发表时间：
2012
影响因子：
5.6
通讯作者：
Hempel N
中科院分区：
文献类型：
--
作者：
Uusitalo LM;Hempel N
活性氧簇(ROS)越来越多地参与细胞信号转导的调控。细胞内ROS通量与细胞功能有关，从细胞增殖到细胞死亡。此外，在局部细胞信号事件期间，ROS微妙的时空变化的重要性正在被认识到。了解所涉及的ROS的生化性质将增强我们对氧化还原信号的了解。因此，理想的细胞内传感器应该能够实时、局部地分辨ROS的变化，对ROS的生理变化高度敏感，并对特定的分子提供特异性。对于体内应用，还需要考虑探针的生物利用度、信号的组织透过率和信噪比等问题。过去，研究人员严重依赖对ROS敏感的荧光探针的使用，最近又使用了基因工程ROS传感器。然而，目前解决上述问题的方法仍有很大的需要改进。最近，分子传感和成像领域已经开始利用纳米颗粒和纳米管独特的物理化学性质。在这里，我们讨论了利用这些纳米结构作为ROS传感的替代平台的最新进展，特别强调细胞内和体内ROS的检测和定量。
Reactive oxygen species (ROS) are increasingly being implicated in the regulation of cellular signaling cascades. Intracellular ROS fluxes are associated with cellular function ranging from proliferation to cell death. Moreover, the importance of subtle, spatio-temporal shifts in ROS during localized cellular signaling events is being realized. Understanding the biochemical nature of the ROS involved will enhance our knowledge of redox-signaling. An ideal intracellular sensor should therefore resolve real-time, localized ROS changes, be highly sensitive to physiologically relevant shifts in ROS and provide specificity towards a particular molecule. For in vivo applications issues such as bioavailability of the probe, tissue penetrance of the signal and signal-to-noise ratio also need to be considered. In the past researchers have heavily relied on the use of ROS-sensitive fluorescent probes and, more recently, genetically engineered ROS sensors. However, there is a great need to improve on current methods to address the above issues. Recently, the field of molecular sensing and imaging has begun to take advantage of the unique physico-chemical properties of nanoparticles and nanotubes. Here we discuss the recent advances in the use of these nanostructures as alternative platforms for ROS sensing, with particular emphasis on intracellular and in vivo ROS detection and quantification.