Salivary gland morphology and virus transmission during long-term cytopathologic West Nile virus infection in Culex mosquitoes
Salivary gland morphology and virus transmission during long-term cytopathologic West Nile virus infection in Culex mosquitoes
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DOI：
10.4269/ajtmh.2007.76.118
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发表时间：
2007-01-01
影响因子：
3.3
通讯作者：
Higgs, Stephen
中科院分区：
文献类型：
--
作者：
Girard, Yvette A.;Schneider, Bradley S.;Higgs, Stephen
在吸血后四周内，通过透射电子显微镜评估了长期西尼罗河病毒（WNV）感染对库蚊唾液腺形态和活力的影响。这些研究表明，细胞凋亡和其他细胞病理学变化更频繁地发生在WNV感染的蚊子相比，未感染的控制。长期感染对西尼罗河病毒传播的影响通过在同一时间段内滴定唾液中的病毒来评估。虽然蚊子唾液中的西尼罗河病毒平均滴度随时间没有显著变化，但含有西尼罗河病毒的唾液样品的百分比下降。由于唾液在吸血和病毒传播中的重要性，唾液腺病理学有可能影响蚊子的摄食行为和病毒传播。这项研究的结果增加了越来越多的证据表明，蚊子媒介中的虫媒病毒感染可能是细胞病变的，并提供了病毒诱导蚊子细胞死亡的潜在机制。
The effect of long-term West Nile virus (WNV) infection on Culex salivary gland morphology and viability was evaluated by transmission electron microscopy during a four week period post-blood feeding. These studies showed that apoptosis and other cytopathologic changes occurred more frequently in WNV-infected mosquitoes compared with uninfected controls. The effect of long-term infection on WNV transmission was evaluated by titering virus in saliva over the same time period. Although the mean titer of WNV in mosquito saliva did not change significantly over time, the percentage of saliva samples containing WNV decreased. Because of the importance of saliva in blood meal acquisition and virus delivery, salivary gland pathology has the potential to affect mosquito feeding behavior and virus transmission. Results from this study add to a growing body of evidence that arbovirus infections in mosquito vectors can be cytopathic, and offer a potential mechanism for virus-induced cell death in mosquitoes.