Endoperoxides Revealed as Origin of the Toxicity of Graphene Oxide.
Endoperoxides Revealed as Origin of the Toxicity of Graphene Oxide.
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DOI：
10.1002/anie.201507070
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发表时间：
2016-01
影响因子：
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通讯作者：
H. Pieper;S. Chercheja;Siegfried Eigler;Christian E Halbig;M. Filipović;A. Mokhir
中科院分区：
文献类型：
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作者：
H. Pieper;S. Chercheja;Siegfried Eigler;Christian E Halbig;M. Filipović;A. Mokhir
氧化石墨烯（GO）的潜在生物医学应用，例如，作为生物分子的载体或用于光热治疗和生物传感的试剂，受到其细胞毒性和致突变性的限制。据信这些特性至少部分是由GO诱导的细胞氧化应激引起的。然而，目前尚不清楚GO的哪些化学片段是造成这种不利影响的原因。我们生成了四个含有可变氧化还原活性基团的表面上，包括Mn（2+），C-中心的自由基，和内过氧化物（EPs）。这些材料在人宫颈癌细胞中产生活性氧的能力的比较表明，EP在GO诱导的氧化应激中起着至关重要的作用。这些数据可用于生物相容性无毒生物医用支架的合理设计。
Potential biomedicinal applications of graphene oxide (GO), for example, as a carrier of biomolecules or a reagent for photothermal therapy and biosensing, are limited by its cytotoxicity and mutagenicity. It is believed that these properties are at least partially caused by GO-induced oxidative stress in cells. However, it is not known which chemical fragments of GO are responsible for this unfavorable effect. We generated four GOs containing variable redox-active groups on the surface, including Mn(2+), C-centered radicals, and endoperoxides (EPs). A comparison of the abilities of these materials to generate reactive oxygen species in human cervical cancer cells revealed that EPs play a crucial role in GO-induced oxidative stress. These data could be applied to the rational design of biocompatible nontoxic GOs for biomedical applications.