Insulin-like growth factor 1 regulates the location, stability, and transcriptional activity of β-catenin
Insulin-like growth factor 1 regulates the location, stability, and transcriptional activity of β-catenin
复制标题
DOI：
10.1073/pnas.210394297
复制
发表时间：
2000-10-24
影响因子：
11.1
通讯作者：
Macaulay, VM
中科院分区：
文献类型：
--
作者：
Playford, MP;Bicknell, D;Macaulay, VM
胰岛素样生长因子（IGF） 1型受体是生长、转化和保护细胞凋亡所必需的。IGFs可以增强细胞迁移，这是通过调节E-cadherin/ β -catenin复合物而受到影响的。我们试图研究igf - 1是否调节人类结直肠癌细胞中E-cadherin和β -catenin之间的相互作用。我们使用的是C10细胞系，我们已经建立了这种细胞系，并且之前已经证明它没有腺瘤性大肠息肉病、e -钙粘蛋白或β -catenin突变。我们发现IGF-1刺激增强了两种蛋白的酪氨酸磷酸化，β -catenin和胰岛素受体底物1，它们与E-cadherin形成复合物。-catenin的酪氨酸磷酸化伴随着快速(
The insulin-like growth factor (IGF) type 1 receptor is required for growth, transformation, and protection from apoptosis. IGFs can enhance cell migration, which is known to be influenced via regulation of the E-cadherin/beta -catenin complex. We sought to investigate whether IGF-l modulated the interaction between E-cadherin and beta -catenin in human colorectal cancer cells. We used the C10 cell line, which we established and have previously shown to lack adenomatous polyposis coli, E-cadherin, or beta -catenin mutations. We found that IGF-1 stimulation enhanced tyrosine phosphorylation of two proteins, beta -catenin and insulin-receptor substrate 1, which formed a complex with E-cadherin. Tyrosine phosphorylation of beta -catenin was accompanied by rapid (