IMMUNOFLUORESCENT LOCALIZATION OF 3 NA,K-ATPASE ISOZYMES IN THE RAT CENTRAL-NERVOUS-SYSTEM - BOTH NEURONS AND GLIA CAN EXPRESS MORE THAN ONE NA,K-ATPASE
IMMUNOFLUORESCENT LOCALIZATION OF 3 NA,K-ATPASE ISOZYMES IN THE RAT CENTRAL-NERVOUS-SYSTEM - BOTH NEURONS AND GLIA CAN EXPRESS MORE THAN ONE NA,K-ATPASE
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DOI：
10.1523/jneurosci.11-02-00381.1991
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发表时间：
1991-02-01
影响因子：
5.3
通讯作者：
SWEADNER, KJ
中科院分区：
文献类型：
--
作者：
MCGRAIL, KM;PHILLIPS, JM;SWEADNER, KJ
在中枢神经系统中，存在钠和钾离子刺激的腺苷三磷酸酶（Na，K-ATP酶）的多种同工酶，其对Na+、ATP和强心苷的亲和力不同。 已知三种形式的催化亚基（指定为α-1，α-2和α-3）来自不同的基因，但对蛋白质的细胞分布或其生理作用知之甚少。 同工酶特异性单克隆抗体允许的免疫荧光定位的3 Na，K-ATP酶在大鼠中枢神经系统中，和显着不同的染色模式。 在1个或多个神经元结构中单独或组合检测到所有3种同工酶，而在神经胶质中检测到α-1和α-2。 许多不同的神经解剖结构或细胞类型被一种以上的同工酶染色。 即使当一个结构或区域染色超过1同工酶，染色模式往往是不同的，这表明基因表达和细胞定位的复杂差异。
In the CNS, there are multiple isozymes of the sodium and potassium ion-stimulated adenosine triphosphatase (Na,K-ATPase) that have differences in affinity for Na+, ATP, and cardiac glycosides. Three forms of the catalytic subunit (designated alpha-1, alpha-2, and alpha-3) are known to be derived from different genes, but little is known of the cellular distributions of the proteins or their physiological roles. Isozyme-specific monoclonal antibodies permitted the immunofluorescent localization of the 3 Na,K-ATPase in the rat CNS, and markedly different patterns of staining were seen. All 3 isozymes were detected, singly or in combination, in 1 or more neuronal structures, while both alpha-1 and alpha-2 were detected in glia. Many different neuroanatomic structures or cell types stained for more than 1 isozyme. Even when a structure or region stained for more than 1 isozyme, the pattern of staining was frequently dissimilar, suggesting complex differences in gene expression and cellular localization.