SYNTHESIS AND CHROMATOGRAPHIC PROPERTIES OF AN HPLC CHIRAL STATIONARY PHASE BASED UPON HUMAN SERUM-ALBUMIN
SYNTHESIS AND CHROMATOGRAPHIC PROPERTIES OF AN HPLC CHIRAL STATIONARY PHASE BASED UPON HUMAN SERUM-ALBUMIN
复制标题
DOI：
10.1007/bf02268706
复制
发表时间：
1990-02-01
期刊：
影响因子：
1.7
通讯作者：
WAINER, IW
中科院分区：
文献类型：
--
作者：
DOMENICI, E;BERTUCCI, C;WAINER, IW
采用原位共价固定化法合成了一种新的高效液相色谱固定相。将该蛋白固定在市售的二醇柱上，二醇柱经1,1-羰基二咪唑活化。初步的色谱研究表明，该相可用于对映溶质的手性分离，这些分离可能反映了与HSA的体外结合。报道了流动相组成和温度对立体化学分辨率的影响。
A new HPLC stationary phase was synthesized by the in situ covalent immobilization of human serum albumin (HSA). The protein was immobilized on a commercially available diol column which had been activated with 1,1-carbonyldiimidazole. Initial chromatographic studies show that this phase can be used for chiral separations of enantiometric solutes and that these separations may reflect in vitro binding to the HSA. The effects of mobile phase composition and temperature on the stereochemical resolutions are reported.