PREDICTIVE MODEL OF CONJUGATIVE PLASMID TRANSFER IN THE RHIZOSPHERE AND PHYLLOSPHERE
PREDICTIVE MODEL OF CONJUGATIVE PLASMID TRANSFER IN THE RHIZOSPHERE AND PHYLLOSPHERE
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DOI：
10.1128/aem.54.2.343-347.1988
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发表时间：
1988-02-01
影响因子：
4.4
通讯作者：
SEIDLER, RJ
中科院分区：
文献类型：
--
作者：
KNUDSEN, GR;WALTER, MV;SEIDLER, RJ
使用计算机模拟模型来预测洋葱假单胞菌（携带可传播重组质粒 R388:Tn1721）与非重组受体菌株在简单根际和叶际微观世界中的存活和接合动态。质粒转移率是针对大规模行动模型得出的，并且供体和受体的存活率被建模为指数增长和衰减过程或两者。速率参数源自实验室研究，其中供体和受体菌株在装有泥炭蛭石溶液的试管中或在培养皿中切下的萝卜或豆叶上孵育。该模型以小时为单位预测供体、受体和转接合子群体。它在种植有萝卜种子并接种了供体和受体菌株的微观世界中以及也在微观世界中生长的萝卜和豆类植物的叶表面上进行了测试。在 7 至 14 天内定期对选择性培养基上的细菌进行计数。当供体和受体群体为106至108 CFU/g（湿重）植物或土壤时，1天后观察到约101至104个转接合体群体。根际和叶表面的转接合子数量最初的增加和随后的减少被正确预测。
A computer simulation model was used to predict the dynamics of survival and conjugation of Pseudomonas cepacia (carrying the transmissible recombinant plasmid R388:Tn1721) with a nonrecombinant recipient strain in simple rhizosphere and phyllosphere microcosms. Plasmid transfer rates were derived for a mass action model, and donor and recipient survival were modeled as exponential growth and decay processes or both. Rate parameters were derived from laboratory studies in which donor and recipient strains were incubated in test tubes with a peat-vermiculite solution or on excised radish or bean leaves in petri dishes. The model predicted donor, recipient, and transconjugant populations in hourly time steps. It was tested in a microcosm planted with radish seeds and inoculated with donor and recipient strains and on leaf surfaces of radish and bean plants also growing in microcosms. Bacteria were periodically enumerated on selective media over 7 to 14 days. When donor and recipient populations were 106 to 108 CFU/g (wet weight) of plant or soil, transconjugant populations of about 101 to 104 were observed after 1 day. An initial increase and a subsequent decline in numbers of transconjugants in the rhizosphere and on leaf surfaces were correctly predicted.