Prediction of transmembrane alpha-helices in prokaryotic membrane proteins: the dense alignment surface method
Prediction of transmembrane alpha-helices in prokaryotic membrane proteins: the dense alignment surface method
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DOI：
10.1093/protein/10.6.673
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发表时间：
1997-06-01
期刊：
影响因子：
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通讯作者：
Elofsson, A
中科院分区：
文献类型：
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作者：
Cserzo, M;Wallin, E;Elofsson, A
已经开发了一种用于预测整合膜蛋白中的跨膜区段的新的简单方法，其基于查询序列针对非同源膜蛋白集合的低严格性点图，使用先前导出的评分矩阵[Cserzo et al.，1994，J. Mol. Biol.生物学：243，388-396]，这种所谓的密集比对表面（DAS）方法显示与早期方法一样执行，早期方法需要多序列比对形式的额外信息或跨膜区段外带正电荷的残基的分布，从而提高了预测能力，序列信息是可用的，或者对于不遵循“阳性内部”规则的膜蛋白类。
A new, simple method for predicting transmembrane segments in integral membrane proteins has been developed, It is based on low-stringency dot-plots of the query sequence against a collection of non-homologous membrane proteins using a previously derived scoring matrix [Cserzo et al., 1994, J. Mol. Biol., 243, 388-396], This so-called dense alignment surface (DAS) method is shown to perform on par with earlier methods that require extra information in the form of multiple sequence alignments or the distribution of positively charged residues outside the transmembrane segments, and thus improves prediction abilities when only single-sequence information is available or for classes of membrane proteins that do not follow the 'positive inside' rule.