Single-molecule recognition imaging-microscopy
Single-molecule recognition imaging-microscopy
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DOI：
10.1073/pnas.0403538101
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发表时间：
2004-08-24
影响因子：
11.1
通讯作者：
Hinterdorfer, P
中科院分区：
文献类型：
--
作者：
Stroh, C;Wang, H;Hinterdorfer, P
原子力显微镜是一种强大且广泛应用的成像技术，它能够在空气和溶液中对单分子进行可视化，并在单分子水平上追踪相关过程。为了在生物应用中发挥最大作用，原子力显微镜需要能够识别图像中特定类型的分子，就像荧光标记在光学显微镜中所起的作用一样。此处呈现的结果表明，高度特异性的抗体 - 抗原相互作用可用于在成分复杂的样品中生成特定类型分子的单分子图谱，同时进行高分辨率的形貌成像。由于它能够识别特定成分，该技术可用于绘制图像上的成分图，并检测过程中发生的成分变化。
Atomic force microscopy is a powerful and widely used imaging technique that can visualize single molecules and follow processes at the single-molecule level both in air and in solution. For maximum usefulness in biological applications, atomic force microscopy needs to be able to identify specific types of molecules in an image, much as fluorescent tags do for optical microscopy. The results presented here demonstrate that the highly specific antibody-antigen interaction can be used to generate single-molecule maps of specific types of molecules in a compositionally complex sample while simultaneously carrying out high-resolution topographic imaging. Because it can identify specific components, the technique can be used to map composition over an image and to detect compositional changes occurring during a process.