Using small molecules to facilitate exchange of bicarbonate and chloride anions across liposomal membranes
Using small molecules to facilitate exchange of bicarbonate and chloride anions across liposomal membranes
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DOI：
10.1038/nchem.178
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发表时间：
2009-05-01
期刊：
影响因子：
21.8
通讯作者：
Quesada, Roberto
中科院分区：
文献类型：
--
作者：
Davis, Jeffery T.;Gale, Philip A.;Quesada, Roberto
碳酸氢盐参与了广泛的生物过程，包括呼吸、细胞内pH调节和受精。在这项研究中，我们使用核磁共振波谱和离子选择电极技术的组合来显示天然产物prodigiiosin，一种由链霉菌和沙雷氏菌等微生物产生的三吡啶分子，促进氯/碳酸氢盐在脂质体膜上的交换（反port）。以4,6-二羟基异眼酰胺核心为基础的高浓度简单合成分子也被证明促进了这一反港过程。虽然众所周知，蛋白质调节细胞中Cl(-)/HCO(3)（-）的交换，但这些结果表明，小分子也可能能够调节生物系统中这些阴离子的浓度。
Bicarbonate is involved in a wide range of biological processes, which include respiration, regulation of intracellular pH and fertilization. In this study we use a combination of NMR spectroscopy and ion-selective electrode techniques to show that the natural product prodigiosin, a tripyrrolic molecule produced by microorganisms such as Streptomyces and Serratia, facilitates chloride/bicarbonate exchange ( antiport) across liposomal membranes. Higher concentrations of simple synthetic molecules based on a 4,6-dihydroxyisophthalamide core are also shown to facilitate this antiport process. Although it is well known that proteins regulate Cl(-)/HCO(3)(-) exchange in cells, these results suggest that small molecules may also be able to regulate the concentration of these anions in biological systems.