EVOLUTION TO PREDOMINANCE OF SWINE INFLUENZA-VIRUS HEMAGGLUTININ MUTANTS OF PREDICTABLE PHENOTYPE DURING SINGLE INFECTIONS OF THE NATURAL HOST
EVOLUTION TO PREDOMINANCE OF SWINE INFLUENZA-VIRUS HEMAGGLUTININ MUTANTS OF PREDICTABLE PHENOTYPE DURING SINGLE INFECTIONS OF THE NATURAL HOST
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DOI：
10.1073/pnas.85.21.8098
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发表时间：
1988-11-01
影响因子：
11.1
通讯作者：
MCGREGOR, S
中科院分区：
文献类型：
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作者：
KILBOURNE, ED;EASTERDAY, BC;MCGREGOR, S
猪流感病毒A/NJ/11/76 H1N1株的L和H2突变体的不同之处在于在病毒的血凝素糖蛋白的153位上具有赖氨酸或谷氨酸。在两个独立的实验中，用不同剂量的H2突变体对猪进行实验性感染，导致20只动物中有11只出现L表型病毒。所有的证据表明，H2 →L突变，选择，并演变为优势发生在7天的跨度内的个人感染。L和H2突变似乎分别作为病毒适应自然宿主和实验室宿主的等位基因。虽然突变体在连续感染过程中的逐渐进化是常见的，但据我们所知，目前对自然宿主中增加的复制效率和特定表型的突变体的快速和可预测的进化的认识是前所未有的。
L and H2 mutants of the A/NJ/11/76 H1N1 strain of swine influenza virus differ by having either a lysine or a glutamic acid at position 153 of the hemagglutinin glycoprotein of the virus. In two separate experiments, experimental infection of swine with various doses of the H2 mutant resulted in the emergence in 11 of 20 animals of virus with the L phenotype. All evidence indicates that the H2 .fwdarw. L mutation, selection, and evolution to predominance occurred within the 7-day span of individual infections. L and H2 mutations appear to act as alleles in the adaptation of virus, respectively, to natural and laboratory hosts. Although the gradual evolution of mutants during sequential infections is commonplace, the present recognition of rapid and predictable evolution of mutants of increased replication efficiency and specific phenotype in the natural host, to our knowledge, is unprecedented.