Methylation Array Analysis of Preoperative and Postoperative Saliva DNA in Crab Cancer Patients
Methylation Array Analysis of Preoperative and Postoperative Saliva DNA in Crab Cancer Patients
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DOI：
10.1158/1055-9965.epi-08-0507
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发表时间：
2008-12-01
影响因子：
3.8
通讯作者：
Schmidt, Brian L.
中科院分区：
文献类型：
--
作者：
Viet, Chi T.;Schmidt, Brian L.
目的：利用口腔鳞状细胞癌 (OSCC) 患者的组织和唾液对 807 个癌症相关基因进行甲基化芯片分析，目的是识别具有诊断和预测价值的生物标志物的高度甲基化基因位点。实验设计：我们对13例OSCC患者术前唾液、术后唾液和组织以及10例正常人唾液中提取的DNA进行了甲基化芯片分析。我们确定了组织和术前唾液样本中高度甲基化的位点，但在术后唾液样本或正常受试者中未甲基化的位点。结果：获得了高质量的 DNA，并且甲基化芯片在所有样本上成功运行。我们发现癌症患者术前和术后唾液的甲基化模式存在显着差异。我们建立了一个由来自 34 个基因的 41 个基因位点组成的基因分类器，这些基因在术前唾液和组织中显示甲基化，但在术后唾液或正常受试者中未甲基化。根据分类器中的基因构建了 4 至 10 个基因的基因组。该小组对 OSCC 的敏感性为 62% 至 77%，特异性为 83% 至 100%。结论：我们报告了口腔癌患者切除前后唾液中 807 个癌症相关基因的甲基化芯片分析。我们的甲基化生物标志物方法证明了唾液甲基化芯片分析可以产生一组特定的癌症相关基因的原理证明，这些基因可以用作口腔癌早期检测的复合生物标志物。 （癌症流行病学生物标记，2008 年以前；17(12):.3603-11）
Purpose: To perform methylation array analysis of 807 cancer-associated genes using tissue and saliva of oral squamous cell carcinoma (OSCC) patients with the objective of identifying highly methylated gene loci that hold diagnostic and predictive value as a biomarker. Experimental Design: We did the methylation array on DNA extracted from preoperative saliva, postoperative saliva, and tissue of 13 patients with OSCC, and saliva of 10 normal subjects. We identified sites that were highly methylated in the tissue and preoperative saliva samples but not methylated in the postoperative saliva samples or in normal subjects.Results: High quality DNA was obtained and the methylation array was successfully run on all samples. We identified significant differences in methylation patterns between the preoperative and postoperative saliva from cancer patients. We established a gene classifier consisting of 41 gene loci from 34 genes that showed methylation in preoperative saliva and tissue but were not methylated in postoperative saliva or normal subjects. Gene panels of 4 to 10 genes were constructed from genes in the classifier. The panels had a sensitivity of 62% to 77% and a specificity of 83% to 100% for OSCC. Conclusions: We report methylation array analysis of 807 cancer-associated genes in the saliva of oral cancer patients before and after oral cancer resection. Our methylation biomarker approach shows the proof of principle that methylation array analysis of saliva can produce a set of cancer-related genes that are specific and can be used as a composite biomarker for the early detection of oral cancer. (Cancer Epidemiol Biornarkers Prev 2008;17(12):.3603-11)