Changes in peripheral blood lymphocyte subpopulations and parasite-specific antibody responses in Trypanosoma evansi infection of sheep
Changes in peripheral blood lymphocyte subpopulations and parasite-specific antibody responses in Trypanosoma evansi infection of sheep
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DOI：
10.1007/s004360050545
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发表时间：
1999-04-01
影响因子：
2
通讯作者：
Luckins, AG
中科院分区：
文献类型：
--
作者：
Onah, DN;Hopkins, J;Luckins, AG
本文报道了感染伊氏锥虫的绵羊外周血淋巴细胞组成的变化。此外，使用双夹心酶联免疫吸附测定 (ELISA) 技术监测寄生虫特异性 IgG(1) 和 IgM 抗体反应。八只羊感染了 2 x 10(6) 伊氏锥虫 TREU 2143，感染的特点是慢性，其中两只羊以自愈告终。这两只羊被指定为 A 组，而另外 6 只羊（在治疗前仍处于寄生虫感染状态）被指定为 B 组。通过间接免疫荧光染色和流式细胞术对外周血淋巴细胞 (PBL) 进行的分析显示，在所有受感染的羊中检测到的 T 细胞和 B 细胞亚群数量发生了显着变化。在A组中，虽然CD8(+)细胞数量减少，但CD4(+)细胞数量略有减少，保持在或高于感染前的数量，导致CD4:CD8比率增加。在B组中，CD8(+)细胞几乎没有出现边际下降，大部分时间等于或高于感染前的数字，而CD4(+)细胞从感染后第26天(p.i.)开始显着下降，使得CD4:CD8比率下降。截至注射后第 26 天，感染还导致显着增加 (P < 0.001)。 B 组循环 B 细胞中 sIg(+)、CD45R(+)、CD1(+) 和主要组织相容性复合体 (MHC) II+ 细胞的数量所示。然而，A 组的增加是中度和双相的。在所有感染的绵羊中均检测到伊氏锥虫特异性 IgM 和 IgG(1) 抗体同种型，但 A 组的水平显着高于 B 组（IgM P < 0.05；IgG(1) P < 0.01）。此外，虽然A组最初较高水平的IgM反应随后被较高水平的IgG(1)反应所取代，但B组直到药物治疗后才出现这种情况。
This paper reports on changes in lymphocyte composition of the peripheral blood in sheep infected with Trypanosoma evansi. In addition, parasite-specific IgG(1) and IgM antibody responses were monitored using a double-sandwich enzyme-linked immunosorbent assay (ELISA) technique. Eight sheep were infected with 2 x 10(6) T. evansi TREU 2143, The infection was characterised by chronicity and ended in self-cure in two of the sheep. These two sheep were designated group A, whereas the other six sheep, which remained parasitaemic until treated, were designated group B. Analysis of the peripheral blood lymphocytes (PBLs) by indirect immunofluorescence staining and flow cytometry revealed significant alterations in the numbers of T- and B-cell subsets detected in all infected sheep. In group A, whereas the numbers of CD8(+) cells decreased, CD4(+) cells showed marginal decreases, remaining at or above pre-infection figures and resulting in increase in the CD4:CD8 ratio. In group B, CD8(+) cells showed few marginal decreases, being at or above preinfection figures most of the time, whereas CD4(+) cells decreased significantly from day 26 post infection (p.i.) such that the CD4:CD8 ratio decreased. Infection also resulted in significant increases (P < 0.001) as of day 26 p.i. in circulating B-cells in group B as shown by the numbers of sIg(+), CD45R(+), CD1(+) and major histocompatibility complex (MHC) II+ cells. The increases, however, were moderate and biphasic in group A. T. evansi-specific IgM and IgG(1) antibody isotypes were detected in all infected sheep, but their levels were significantly higher in group A than in group B (IgM P < 0.05; IgG(1) P < 0.01). In addition, although an initially higher level of IgM response was subsequently replaced by a higher level of IgG(1) response in group A, this was never the case in group B until after drug treatment.