Expression and localization of IL-1β mRNA is interrelated with cytoskeletal rearrangement in monocytes stimulated by adherence:: A light microscopy in situ hybridization study
Expression and localization of IL-1β mRNA is interrelated with cytoskeletal rearrangement in monocytes stimulated by adherence:: A light microscopy in situ hybridization study
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DOI：
10.1046/j.1440-1711.2001.01031.x
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发表时间：
2001-09-01
影响因子：
4
通讯作者：
Watson, JM
中科院分区：
文献类型：
--
作者：
Böcker, U;Sirenko, OI;Watson, JM
非贴壁单核细胞和受贴壁刺激的单核细胞之间 IL-1 β mRNA 表达、稳定性和翻译的差异表明细胞因子的调节与细胞骨架结构的功能和组装有关。进行原位杂交研究以可视化贴壁培养的单核细胞中 IL-1 β mRNA 的表达和定位。 IL-1 beta mRNA 表达具有异质性，在扩散或极化细胞中发现高转录水平。转录本被划分到扩散细胞的核周区域，并通过极化部分重新分布。与其他运动细胞群中的 mRNA 分布相反，IL-1 β mRNA 并不定位于远端或近端肌动蛋白细胞骨架。转录本的核周限制需要完整的肌动蛋白微丝。细胞骨架破坏和去污剂提取处理表明大多数非翻译 IL-1 β mRNA 与中间丝相关。在 LPS 刺激的单核细胞中，IL-1β（而非 IL-1Ra）转录物重新分布并部分关联，但不与肌动蛋白微丝结合。本研究表明，贴壁单核细胞中 IL-1 β mRNA 的表达和定位与贴壁、扩散和极化时的细胞骨架重排相关。
Differences in IL-1 beta mRNA expression, stability and translation between non-adherent monocytes and those stimulated by adherence suggest that cytokine regulation is coupled to the function and assembly of cytoskeletal structures. In situ hybridization studies were performed to visualize expression and positioning of IL-1 beta mRNA in adherently cultivated monocytes. IL-1 beta mRNA expression was heterogeneous with high transcript levels found in spread or polarized cells. Transcripts were compartmentalized to the perinuclear region in spread cells, and partially redistributed with polarization. In contrast to mRNA distribution in other motile cell populations, IL-1 beta mRNA did not localize to the distal or proximal actin cytoskeleton. Perinuclear confinement of transcripts required intact actin microfilaments. Treatment with cytoskeleton disruption and detergent extraction suggested that most non-translated IL-1 beta mRNA was associated with intermediate filaments. In monocytes stimulated by LPS, IL-1 beta, but not IL-1Ra transcripts were redistributed and partially associated, yet not bound to actin microfilaments. The present study demonstrates that IL-1 beta mRNA expression and localization in adherent monocytes is interrelated with the cytoskeletal rearrangement upon adherence, spreading and polarization.