Thymosin 4 Prevents Angiotensin II-Induced Cardiomyocyte Growth by Regulating Wnt/WISP Signaling
Thymosin 4 Prevents Angiotensin II-Induced Cardiomyocyte Growth by Regulating Wnt/WISP Signaling
复制标题
胸腺肽 4 通过调节 Wnt/WISP 信号传导防止血管紧张素 II 诱导的心肌细胞生长
DOI：
10.1002/jcp.25275
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发表时间：
2016-08-01
影响因子：
5.6
通讯作者：
Gupta, Sudhiranjan
中科院分区：
文献类型：
--
作者：
Li, Li;Guleria, Rakeshwar S.;Gupta, Sudhiranjan
胸腺素-4 （T4）是一种普遍存在的蛋白，具有许多与细胞增殖和分化有关的特性，促进伤口愈合和调节炎症介质。然而，T4在心肌细胞肥大中的作用目前尚不清楚。本研究的目的是确定T4对血管紧张素II （Ang II）诱导的心肌细胞生长的心脏保护作用。用T4预处理新生大鼠心室心肌细胞（NRVM），然后再用Ang II刺激。采用实时荧光定量PCR、Western blotting和荧光显微镜检测细胞大小、肥大标记基因表达和Wnt信号成分-catenin、Wnt诱导分泌蛋白-1 （WISP-1）。T4预处理导致细胞大小、肥大标记基因和wnt相关基因表达及蛋白水平降低；由angii诱导的心肌细胞。在NRVM中过表达WISP-1，并评价T4对Ang ii诱导的心肌细胞生长的影响。WISP-1过表达促进心肌细胞生长，并通过T4预处理逆转。这是第一个证明T4靶向Wnt/WISP-1以保护Ang ii诱导的心肌细胞生长的报道。j .细胞。中国生物医学工程学报，2016,31(2):444 - 444。(c) 2015 Wiley期刊，inc .胸腺素β 4 (T4)， g -肌动蛋白隔离蛋白靶向Wnt/WISP-1保护angii诱导的心肌细胞生长。
Thymosin beta-4 (T4) is a ubiquitous protein with many properties relating to cell proliferation and differentiation that promotes wound healing and modulates inflammatory mediators. However, the role of T4 in cardiomyocyte hypertrophy is currently unknown. The purpose of this study was to determine the cardio-protective effect of T4 in angiotensin II (Ang II)-induced cardiomyocyte growth. Neonatal rat ventricular cardiomyocytes (NRVM) were pretreated with T4 followed by Ang II stimulation. Cell size, hypertrophy marker gene expression and Wnt signaling components, -catenin, and Wnt-induced secreted protein-1 (WISP-1) were evaluated by quantitative real-time PCR, Western blotting and fluorescent microscopy. Pre-treatment of T4 resulted in reduction of cell size, hypertrophy marker genes and Wnt-associated gene expression, and protein levels; induced by Ang II in cardiomyocyte. WISP-1 was overexpressed in NRVM and, the effect of T4 in Ang II-induced cardiomyocyte growth was evaluated. WISP-1 overexpression promoted cardiomyocytes growth and was reversed by pretreatment with T4. This is the first report which demonstrates that T4 targets Wnt/WISP-1 to protect Ang II-induced cardiomyocyte growth. J. Cell. Physiol. 231: 1737-1744, 2016. (c) 2015 Wiley Periodicals, Inc.Thymosin beta 4 (T4), G-actin sequestering protein targets Wnt/WISP-1 to protect Ang II-induced cardiomyocyte growth.