Inverse Association Between the Skin and Oral Microbiota in Atopic Dermatitis
Inverse Association Between the Skin and Oral Microbiota in Atopic Dermatitis
复制标题
特应性皮炎中皮肤和口腔微生物群之间的负相关。
DOI：
10.1016/j.jid.2019.02.009
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发表时间：
2019-08-01
影响因子：
6.5
通讯作者：
Yao, Xu
中科院分区：
文献类型：
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作者：
Li, Wei;Xu, Xiaoqiang;Yao, Xu
先前的研究已经独立地表明，皮肤和肠道微生物群与特应性皮炎（AD）密切相关;然而，AD患者不同栖息地的微生物群作为一个综合群落尚未得到表征。本研究通过16 S核糖体RNA基因扩增子测序，比较分析了172例AD患者和120例健康对照者皮肤、口腔和肠道微生物群落的结构和功能。AD患者的皮肤和口腔（而非肠道）表现出微生物多样性的差异性减少，并且这些微生物多样性与疾病严重程度明显相关。在不同的栖息地中发现了不同程度的社区结构的变化，AD患者的皮肤和口腔微生物群之间的谱系距离比对照组中观察到的更近。AD患者的不同栖息地在属水平上表现出位点特异性改变，许多AD的口腔特异性微生物在皮肤和口腔中表现出相反的富集方向。最有趣的是，在AD患者的皮肤和口腔微生物群之间发现了功能途径的负相关。此外，AD患者不同身体部位的微生物群变化受年龄的影响不同。
Previous studies have shown independently that the skin and gut microbiota are closely associated with atopic dermatitis (AD); however, the microbiota across different habitats of AD patients as an integrated community has not been characterized. In the present study, we comparatively analyzed the structure and function of the microbial communities in the skin, oral cavity, and gut of 172 AD patients and 120 healthy controls through 16S ribosomal RNA gene amplicon sequencing. The skin and oral cavity, but not the gut, of AD patients demonstrated differential reduction in the microbial diversity, and these were distinctly correlated with disease severity. Different degrees of shifts in the community structure were found among different habitats, and the lineage distance between the skin and oral microbiota of AD patients was closer than that observed in the controls. The different habitats of AD patients exhibited site-specific alterations at the genus level, and many oral-specific microbes of AD showed opposing directions of enrichment in the skin and oral cavity. Most interestingly, an inverse association in the functional pathways was found between the skin and oral microbiota of AD patients. Additionally, the alterations of the microbiota in different body sites of AD patients were differentially affected by age.