Moderate exercise changes synaptic and cytoskeletal proteins in motor regions of the rat brain
Moderate exercise changes synaptic and cytoskeletal proteins in motor regions of the rat brain
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DOI：
10.1016/j.brainres.2010.09.045
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发表时间：
2010-11-18
期刊：
影响因子：
2.9
通讯作者：
Britto, Luiz R. G.
中科院分区：
文献类型：
--
作者：
Ferreira, Ana F. B.;Real, Caroline C.;Britto, Luiz R. G.
众所周知，体育锻炼可以在多个方面增强大脑功能。我们评估了适度强迫运动方案对大鼠大脑区域中与运动功能相关且经常受神经退行性疾病影响的突触蛋白（即突触蛋白 1 (SYN) 和突触素 (SYP)）以及结构蛋白（神经丝，NF）的急性影响。使用免疫组织化学、蛋白质印迹和实时 PCR 分析运动 3、7 和 15 天后这些蛋白的表达（EX3、EX7 和 EX15）。在小脑中，在 EX7 和 EX15 处观察到 SYN 增加，在 EX3 处观察到 NF68 增加。在黑质中，在 EX3 处观察到 NF68 和 NF160 的蛋白质水平增加。在纹状体中，EX3 和 EX7 处的 SYN 增加，EX7 处的 SYP 增加，EX3 处的 NF68 增加。在皮质中，在 EX3 处观察到 NF68 和 NF160 水平降低，随后在 EX15 处观察到 NF68 水平增加。在网状结构中，所有 NF 蛋白在 EX15 时均增加。每个时间点和区域的 mRNA 数据还揭示了 SYN、SYP 和 NF 表达的显着运动相关变化。这些结果表明，适度的体育锻炼可以调节运动脑区域的突触和结构蛋白，这可能在运动依赖性大脑可塑性中发挥重要作用。 (C) 2010 Elsevier B.V. 保留所有权利。
Physical exercise is known to enhance brain function in several aspects. We evaluated the acute effects of a moderate forced exercise protocol on synaptic proteins, namely synapsin 1 (SYN) and synaptophysin (SYP), and structural proteins (neurofilaments, NFs) in rat brain regions related to motor function and often affected by neurodegenerative disorders. Immunohistochemistry, Western blotting and real-time PCR were used to analyze the expression of those proteins after 3, 7 and 15 days of exercise (EX3, EX7 and EX15). In the cerebellum, increase of SYN was observed at EX7 and EX15 and of NF68 at EX3. In the substantia nigra, increases of protein levels were observed for NF68 and NF160 at EX3. In the striatum, there was an increase of SYN at EX3 and EX7, of SYP at EX7 and of NF68 at EX3. In the cortex, decreased levels of NF68 and NF160 were observed at EX3, followed by an increase of NF68 at EX15. In the reticular formation, all NF proteins were increased at EX15. The mRNA data for each time-point and region also revealed significant exercise-related changes of SYN, SYP and NF expression. These results suggest that moderate physical exercise modulates synaptic and structural proteins in motor brain areas, which may play an important role in the exercise-dependent brain plasticity. (C) 2010 Elsevier B.V. All rights reserved.