POLY I-C POLY-L-LYSINE - POTENT INDUCER OF INTERFERONS IN PRIMATES
POLY I-C POLY-L-LYSINE - POTENT INDUCER OF INTERFERONS IN PRIMATES
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DOI：
10.1089/jir.1981.1.539
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
HILLEMAN, MR
中科院分区：
文献类型：
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作者：
LAMPSON, GP;FIELD, AK;HILLEMAN, MR
聚I：C/聚L-赖氨酸(聚I：C/聚L-赖氨酸)的可溶性络合物可诱导猕猴体内产生高滴度的干扰素。通过限制赖氨酸和核苷酸的摩尔比为0.5，形成了一种可溶于2.0 mg Poly I：C/ml的磷酸盐缓冲盐水的络合物。研究了不同相对分子质量的聚I：C/聚L赖氨酸络合物在恒定的赖氨酸与核苷酸比例(0.5)下诱导猕猴血清干扰素的能力。使用聚I：C/聚L赖氨酸MW-gtoreq络合物，观察到聚I：C在灰猴体内诱导干扰素的能力显著增强(10-100倍)。104个道尔顿。聚I：C/聚L赖氨酸是猕猴、恒河猴、黑猩猩和绒猴体内干扰素的有效诱导剂。通过每天重复给药，灰猴体内的干扰素效价(100U/ml血液)保持在较高水平。在几个月的重复诱导后，没有注意到长期的低反应。猴血清对聚I：C/聚L赖氨酸的反应产生的干扰素生物学特性与人白细胞干扰素相似。
A soluble complex of poly I:C and poly-L-lysine (poly I:C/poly-L-lysine) induced high titers of circulating interferon in monkeys. By limiting the molar ratio of lysine to nucleotide to 0.5, a complex was formed that was soluble up to 2.0 mg poly I:C/ml of phosphate-buffered saline. Poly I:C/poly-L-lysine complexes of various MW, and with a constant ratio (0.5) of lysine to nucleotide, were evaluated for capacity to induce serum interferon in grivet monkeys. Substantial enhancement (10- to 100-fold) of the capacity of poly I:C to induce interferon in grivet monkeys was observed using poly I:C/poly-L-lysine complexes of MW .gtoreq. 104 daltons. The poly I:C/poly-L-lysine was an effective inducer of interferon in grivet monkeys, rhesus monkeys, chimpanzees and marmosets. A high interferon titer (> 100 U/ml blood) was maintained in grivet monkeys by repeated daily administration of the complex. No long-term hyporesponsiveness was noted following repeat inductions over a period of months. The serum interferon produced in monkeys in response to poly I:C/poly-L-lysine resembled human leukocyte interferon in its biological characteristics.