IONIC PERMEABILITY OF SARCOPLASMIC-RETICULUM VESICLES MEASURED BY LIGHT-SCATTERING METHOD
IONIC PERMEABILITY OF SARCOPLASMIC-RETICULUM VESICLES MEASURED BY LIGHT-SCATTERING METHOD
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DOI：
10.1007/bf01871994
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发表时间：
1978-01-01
影响因子：
2.4
通讯作者：
KASAI, M
中科院分区：
文献类型：
--
作者：
KOMETANI, T;KASAI, M
[兔肌肉]肌浆网囊泡的体积变化，随后通过测量光散射强度。当肌浆网囊泡悬浮液的盐浓度通过使用停流装置增加时，光散射强度在开始时迅速增加，然后降低。光散射强度的快速增加是由于水的流出导致肌浆网囊泡体积的减少而引起的。随后的光散射强度的降低是由于溶质和水的流入导致的体积增加而引起的。由前者和后者的渗透速率，可以分别计算水和溶质的渗透时间。根据相同的方法，测定了各种盐的渗透时间。盐的流入速率取决于较慢离子的运动，即，离子成对移动。在K盐的情况下，当缬氨霉素加入到膜悬浮液中时，观察到盐的渗透速率增加。从这些实验中，作为渗透性的量度，测定各种离子和分子的半渗透时间。以下是典型的结果：水0.1，Li+ 36，Na+ 26，K+ 20，Rb+ 16，Cl- 0.4，甲磺酸盐20，磷酸盐10.5，草酸盐40，在室温下几秒钟内。肌浆网作为一个整体，被发现是一个阴离子渗透膜。
The volume change of [rabbit muscle] sarcoplasmic reticulum vesicles was followed by measuring the light scattering intensity. When the salt concentration of the suspension of sarcoplasmic reticulum vesicles was increased by using a stopped flow apparatus, the light scattering intensity rapidly increased at the beginning and then decreased. The fast increase in the light scattering intensity is caused by the decrease of the volume of sarcoplasmic reticulum vesicles due to the outflow of water. The following decrease in the light scattering intensity is caused by the increase of the volume due to the inflow of the solutes and water. From the former and the latter rates, the permeation times of water and the solutes could be calculated, respectively. According to the same method, permeation times of various salts were determined. The rate of the inflow of the salts was dependent on the movement of the slower ions, i.e., ions move as a pair. In the case of K salts, an increase in the permeation rate of the salts was observed when valinomycin was added to the membrane suspensions. From these experiments, as a measure of permeability, half permeation times of various ions and molecules were determined. The following are typical results: water 0.1, Li+ 36, Na+ 26, K+ 20, Rb+ 16, Cl- 0.4, methanesulfonate 20, phosphate 10.5, oxalate 40 in seconds at room temperature. As a whole, sarcoplasmic reticulum was found to be an anion permeable membrane.