Structure of transfer RNA by carbon NMR: resolution of single carbon resonances from 13C-enriched, purified species.
Structure of transfer RNA by carbon NMR: resolution of single carbon resonances from 13C-enriched, purified species.
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通过碳 NMR 分析转移 RNA 的结构：从富含 13C 的纯化物种中解析单碳共振。
DOI：
10.1093/nar/8.9.2085
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发表时间：
1980
影响因子：
14.9
通讯作者：
Schmidt,PG
中科院分区：
文献类型：
--
作者：
Agris,PF;Schmidt,PG
首次获得了纯化tRNA的甲基碳-13核磁共振谱。此外，苯丙氨酸、酪氨酸和半胱氨酸特异性tRNA的光谱表现出单甲基碳共振的首次分辨率。碳-13富集的核糖体嘧啶(T)和7-甲基鸟苷（m7g）甲基基团和2-甲基硫代- n6 -（δ2-异戊烯基）腺苷（ms216A）甲基硫基被分离。tRNAPhe的T甲基信号从添加Mg2+ 450时的12.3 ppm转变为添加10mM MgCl2时的11.1 ppm。同样的条件变化导致m7G甲基信号向相反方向偏移0.4 ppm。相对容易获得纯化tRNA物种的单碳共振，以及显示其化学变化对局部结构变化的敏感性，是13c - nmr在探测tRNA构象及其与蛋白质和其他核酸相互作用时变化的有用技术的必要标准。
Methyl carbon-13 NMR spectra of purified tRNA species are presented for the first time. In addition, these spectra of tRNA species specific for phenylalanine, tyrosine, and cysteine exhibited the first resolution of single methyl carbon resonances. Carbon-13 enriched methyl groups of ribothymidine (T) and 7-methylguanosine (m7g) and the methylthio group of 2-methylthio-N6-(δ2-isopentenyl) adenosine (ms216A) were resolved. The T methyl signal of tRNAPhe shifted from 12.3 ppm at 450 in the absence of added Mg2+to 11.1 ppm at 300 in the presence of 10mM MgCl2. The same change in conditions led to a 0.4 ppm shift of the m7G methyl signal in the opposite direction. The relative ease in obtainment of single carbon resonances of purified tRNA species, and display of the sensitivity of their chemical shifts to changes in local structure, are requisite criteria for13C-NMR to be a useful technique in probing tRNA conformation and its changes during interaction with proteins and other nucleic acids.