Cellular origin of endochondral ossification from grafted periosteum
Cellular origin of endochondral ossification from grafted periosteum
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移植骨膜软骨内骨化的细胞起源
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
Toshio Yamamoto
中科院分区：
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作者：
T. Ueno;T. Kagawa;N. Mizukawa;Hiroaki Nakamura;T. Sugahara;Toshio Yamamoto
移植的骨膜有可能通过软骨内骨化形成异位骨。虽然骨软骨形成细胞被认为起源于移植骨膜的成骨层，但还没有组织学报告证明这一点。本研究旨在阐明移植骨膜中骨形成前软骨形成的起源。将取自日本白色幼兔胫骨的骨膜移植到舌骨上肌中，并在术后第1、7、9、14、21和35天进行X线和组织学检查。正常骨膜和胫骨移植部位也进行了检查。手术获取的骨膜分裂和破坏其成骨层，但保留完整的纤维层。大部分的成骨细胞保留在胫骨表面，只有少量的成骨层细胞存在于移植组织中。嫁接后第7天，纤维层已增厚。纤维层成纤维细胞明显增多（P < 0.01），增殖细胞核抗原染色阳性。这些细胞在第9天表现出碱性磷酸酶活性。分化的软骨细胞在术后14天形成软骨。成骨细胞层出现坏死，随后消失。术后第21天，软骨被骨小梁取代。骨形成在35天内完成。此时的X线分析也显示了新骨形成。这些发现表明，移植骨膜通过软骨内骨化形成骨，并且纤维层的细胞在这种骨形成之前的软骨形成中起重要作用。Anat Rec 264：348-357，2001年。© 2001 Wiley利斯公司
Grafted periosteum is known to have potential for heterotopic bone formation by endochondral ossification. Although osteochondrogenic cells have been thought to originate from the osteogenic layer in grafted periosteum, no histological report has yet demonstrated this. The present study was designed to elucidate the origin of chondrogenesis preceding bone formation in grafted periosteum. Periostea harvested from young Japanese white rabbits' tibiae were grafted into suprahyoid muscles and examined radiographically and histologically at postoperative days 1, 7, 9, 14, 21, and 35. Normal periostea and tibial graft site were also examined. Surgical harvesting of the periosteum split and damaged its osteogenic layer but retained the fibrous layer intact. Most of the osteoblasts remained on the tibial bone surface, and only few cells of the osteogenic layer were present in grafted tissue. By the seventh day after grafting, the fibrous layer had thickened. The fibroblastic cells in the fibrous layer had significantly increased in number (P < 0.01) and were positively stained for proliferating cell nuclear antigen. These cells exhibited alkaline phosphatase activity at day 9. The differentiated chondrocytes had formed cartilage at postoperative day 14. Cells in the osteogenic layer appeared necrotic and subsequently disappeared. Following postoperative day 21, cartilage was replaced by trabecular bone. Bone formation was completed by 35 days. An X‐ray analysis at this time also revealed new bone formation. These findings indicate that grafted periosteum forms bone by endochondral ossification and that the cells of the fibrous layer play essential roles in chondrogenesis that precedes such bone formation. Anat Rec 264:348–357, 2001. © 2001 Wiley‐Liss, Inc.