Drug delivery in pluronic micelles: effect of high-frequency ultrasound on drug release from micelles and intracellular uptake
Drug delivery in pluronic micelles: effect of high-frequency ultrasound on drug release from micelles and intracellular uptake
复制标题
DOI：
10.1016/s0168-3659(02)00262-6
复制
发表时间：
2002-11-07
影响因子：
10.8
通讯作者：
Rapoport, NY
中科院分区：
文献类型：
--
作者：
Marin, A;Sun, H;Rapoport, NY
研究了高频超声对早幼粒细胞白血病HL-60细胞、卵巢癌药物敏感和多药耐药细胞(A2780和A2780/ADR)以及乳腺癌MCF-7细胞的多柔比星(DOX)从Pluronic胶束释放和细胞内摄取的影响。用自由基捕获法记录了高频超声引发的空化事件。在比低频超声(20-100 kHz)高得多的功率密度下，观察到瞬时空化的开始和DOX从胶束中的释放，即使是短时间(15-30 S)的高频超声波也显著增加了细胞内从PBS、RPMI1640和Pluronic胶束中的DOX摄取。对观察到的效应的机理进行了讨论。(C)2002 Elsevier Science B.V.保留所有权利。
The effect of high-frequency ultrasound oil doxorubicin (DOX) release from Pluronic micelles and intracellular DOX uptake was studied for promyelocytic leukemia HL-60 cells, ovarian carcinoma drug-sensitive and multidrug-resistant (MDR) cells (A2780 and A2780/ADR, respectively), and breast cancer MCF-7 cells. Cavitation events initiated by high-frequency ultrasound were recorded by radical trapping. The onset of transient cavitation and DOX release from micelles were observed at much higher power densities than at low-frequency ultrasound (20-100 kHz), Even a short (15-30 s) exposure to high-frequency ultrasound significantly enhanced the intracellular DOX uptake from PBS, RPMI 1640, and Pluronic micelles. The mechanism,, of the observed effects are discussed. (C) 2002 Elsevier Science B.V. All rights reserved.