TRANSFER OF RESISTANCE PLASMIDS FROM STAPHYLOCOCCUS-EPIDERMIDIS TO STAPHYLOCOCCUS-AUREUS - EVIDENCE FOR CONJUGATIVE EXCHANGE OF RESISTANCE
TRANSFER OF RESISTANCE PLASMIDS FROM STAPHYLOCOCCUS-EPIDERMIDIS TO STAPHYLOCOCCUS-AUREUS - EVIDENCE FOR CONJUGATIVE EXCHANGE OF RESISTANCE
复制标题
DOI：
10.1128/jb.153.2.627-634.1983
复制
发表时间：
1983-01-01
影响因子：
3.2
通讯作者：
SCHABERG, DR
中科院分区：
文献类型：
--
作者：
FORBES, BA;SCHABERG, DR
通过在滤膜上混合培养来测试表皮葡萄球菌将抗菌素耐药性转移至金黄色葡萄球菌的能力。检查的 6 个临床分离株中有两个能够转移对金黄色葡萄球菌菌株 879R4RF、RN450RF 和 UM1385RF 的耐药性。随后与表皮葡萄球菌供体交配产生的金黄色葡萄球菌转接合体能够以相似的频率充当其他金黄色葡萄球菌菌株的供体。供体和转接合子的无细胞和丝裂霉素 C 诱导的滤液没有显示出噬斑形成能力。在交配混合物和琼脂中添加 DNAse I、柠檬酸盐、EDTA、氯化钙和人血清对转移没有影响。无法存活的供体细胞无法转移抗性，且在 4°C 时未发生转移。 C. 需要细胞与细胞之间的接触，因为转移不会在肉汤中发生，或者当供体和受体的过滤器分别背靠背放置时，细胞不会直接接触。对表皮葡萄球菌分离株 UM899、其随后的金黄色葡萄球菌转接合体和固化衍生物的 DNA 分析表明，所有转移的抗性标记均驻留在质粒上。交配实验表明庆大霉素质粒 pAM899-1 在转移过程中发挥着核心作用。结果与从表皮葡萄球菌到金黄色葡萄球菌的抗性接合转移一致，类似于在链球菌物种中针对诸如pAM.beta.1的质粒所证明的质粒转移。这代表了葡萄球菌之间基因交换的新机制。
The ability of S. epidermidis to transfer antimicrobial resistance to S. aureus was tested by mixed culture on filter membranes. Two of 6 clinical isolates examined were able to transfer resistance to S. aureus strains 879R4RF, RN450RF and UM1385RF. Subsequent S. aureus transconjugants resulting from matings with S. epidermidis donors were able to serve as donors to other S. aureus strains at similar frequencies. Cell-free and mitomycin C-induced filtates of donors and transconjugants showed no plaque-forming ability. Addition of DNAse I, citrate, EDTA, calcium chloride and human sera to mating mixes and agar showed no effect on transfer. Nonviable donor cells were unable to transfer resistance and transfer did not occur at 4.degree. C. Cell-to-cell contact was required since transfer did not occur in broth or when filters of donor and recipient, respectively, were placed back-to-back so cells were not in direct contact. Analysis of DNA from S. epidermidis isolate UM899, its subsequent S. aureus transconjugants and cured derivatives demonstrated that all resistance markers which transferred resided on plasmids. Mating experiments suggested a central role for the gentamicin plasmid pAM899-1 in the transfer process. The results are consistent with a conjugative transfer of resistance from S. epidermidis to S. aureus analogous to plasmid transfer demonstrated in streptococcal species for plasmids such as pAM.beta.1. This represents a novel mechanism for gene exchange among staphylococci.