Overexpression of TsApx1 from Thellungiella salsuginea improves abiotic stress tolerance in transgenic Arabidopsis thaliana
Overexpression of TsApx1 from Thellungiella salsuginea improves abiotic stress tolerance in transgenic Arabidopsis thaliana
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来自 Thellungiella salsuginea 的 TsApx1 过表达提高了转基因拟南芥的非生物胁迫耐受性
DOI：
10.1007/s10535-015-0533-y
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发表时间：
2015-07
影响因子：
1.5
通讯作者：
Zhang, Genfa
中科院分区：
文献类型：
--
作者：
Li, Jingxiao;Li,Hongjie;Shi, Zihan;Zhang, Genfa
盐生植物Thellungiella salsuginea因其基因组小、生命周期短、种子产量丰富而成为一种新的模式植物。尽管 T. salsuginea 与其近亲拟南芥具有较高的序列同一性，但它对盐度、干旱、冰冻、炎热和寒冷表现出更强的耐受性。为了阐明拟南芥的非生物胁迫抗性机制，我们对其胞质 Apx1 基因（TsApx1）进行了表征，并建立了过表达 TsApx1 的拟南芥转基因系。在300 mM NaCl条件下，过表达TsApx1（35S：TsApx1-GFP）的转基因植株中H2O2、丙二醛和脯氨酸的含量较低，超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和抗坏血酸过氧化物酶的活性均高于野生型植株。拟南芥的 atapx1 突变体植物具有 NaCl/甘露醇敏感表型。 atapx1突变体中TsApx1的异位表达有效地纠正了表型。这些结果表明，TsApx1 在盐度或干旱条件下清除细胞质中的活性氧方面发挥着重要作用。尽管 TsApx1 在 T. salsuginea 中持续高水平表达，但该基因显然是可诱导的。总之，TsApx1的高组成型表达和快速诱导可能有助于T. salsuginea对非生物胁迫的耐受性。
The halophyte Thellungiella salsuginea is a new model plants due to its small genome size, short life cycle, and copious seed production. Although T. salsuginea shares a high sequence identity with its close relative Arabidopsis thaliana, it shows a greater tolerance to salinity, drought, freezing, heat, and cold. To elucidate the mechanism of abiotic stress resistance in T. salsuginea, we characterized its cytosolic Apx1 gene (TsApx1) and established A. thaliana transgenic lines overexpressing TsApx1. Under 300 mM NaCl, the content of H2O2, malondialdehyde, and proline were lower and the activities of superoxide dismutase, catalase, glutathione peroxidase, and ascorbate peroxidase were all higher in the transgenic plants overexpressing TsApx1 (35S:TsApx1-GFP) than in the wild-type plants. The atapx1 mutant plants of A. thaliana had a NaCl/mannitol-sensitive phenotype. The ectopic expression of TsApx1 in the atapx1 mutant effectively remedied the phenotype. These results suggest that TsApx1 plays an important role in scavenging reactive oxygen species in the cytoplasm under salinity or drought. Although TsApx1 in T. salsuginea was constantly expressed at a high level, this gene was clearly inducible. In summary, the high constitutive expression and rapid induction of TsApx1 may contribute to the tolerance to abiotic stresses in T. salsuginea.
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拟南芥植物在延长的热应激条件下增强了种子的产生，缺乏胞质抗坏血酸过氧化物酶2。
影响因子：
6.9
作者：
Suzuki N;Miller G;Sejima H;Harper J;Mittler R
通讯作者：
Mittler R
影响因子：
4.3
作者：
Panchuk, II;Zentgraf, U;Volkov, RA
通讯作者：
Volkov, RA
影响因子：
7.4
作者：
Miller, Gad;Suzuki, Nobuhiro;Mittler, Ron
通讯作者：
Mittler, Ron
影响因子：
11.6
作者：
Davletova, S;Rizhsky, L;Mittler, R
通讯作者：
Mittler, R
影响因子：
4.1
作者：
Kangasjarvi, Saijaliisa;Lepisto, Anna;Rintamaki, Eevi
通讯作者：
Rintamaki, Eevi