Molecular cloning and preliminary genetic analysis of the men gene cluster of Bacillus subtilis.
Molecular cloning and preliminary genetic analysis of the men gene cluster of Bacillus subtilis.
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枯草芽孢杆菌men基因簇的分子克隆及初步遗传分析。
DOI：
10.1128/jb.170.6.2735-2741.1988
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发表时间：
1988
影响因子：
3.2
通讯作者：
Taber,H
中科院分区：
文献类型：
--
作者：
Miller,P;Rabinowitz,A;Taber,H
通过使用可整合的质粒载体克隆含有编码甲萘醌生物合成酶的基因（men基因）的枯草芽孢杆菌染色体基因座。在三个重叠的重组质粒上重建了男性簇，并推导出一个暂定的基因顺序。转录方向和转录边界的评估表明，男性基因表达的形式至少有一个多顺反子的信息。此外，一个频谱的男人表型的不同的内部片段的集群的整合表明转录的复杂性，可能包括一个内部启动子。包含转录单位所需的克隆DNA片段的大小，以及这些片段中已知的men突变的位置，表明先前未与men系统鉴定的基因也位于该簇内。克隆的men序列提供了探针，用于检测men基因座响应于变化的环境条件和在以内生孢子形成为代表的发育序列期间的转录模式。
The Bacillus subtilis chromosomal locus that contains the genes encoding the menaquinone biosynthetic enzymes (the men genes) was cloned by using an integrable plasmid vector. The men cluster was reconstituted on three overlapping recombinant plasmids, and a tentative gene order was derived. Evaluations of the direction of transcription and of transcriptional boundaries suggested that the men genes are expressed in the form of at least one polycistronic message. In addition, a spectrum of Men phenotypes resulting from the integration of different internal fragments of the cluster indicate transcriptional complexities, possibly including an internal promoter. The size of cloned DNA fragments required to encompass the transcription unit, as well as the locations of known men mutations within these fragments, suggests that a gene(s) not previously identified with the men system is also located within the cluster. The cloned men sequences make available probes to examine the patterns of transcription from the men locus in response to changing environmental conditions and during the developmental sequence represented by endospore formation.