Expressed sequence tag analysis of functional genes associated with adventitious rooting in Liriodendron hybrids.
Expressed sequence tag analysis of functional genes associated with adventitious rooting in Liriodendron hybrids.
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DOI：
10.4238/gmr.15027606
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发表时间：
2016-06
期刊：
影响因子：
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通讯作者：
Zhong Yd;Sun Xy;Liu Ey;Li Yq;Z. Gao;Yu Fx
中科院分区：
文献类型：
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作者：
Zhong Yd;Sun Xy;Liu Ey;Li Yq;Z. Gao;Yu Fx
鹅掌楸杂交种（Liriodendron chinense x L. tulipifera）是重要的园林绿化和造林硬木树种。迄今为止，关于这些杂种以及鹅掌楸属的不定根的基因组研究报道很少。在本研究中，我们利用不定根构建了第一个鹅掌楸杂种的 cDNA 文库。总共生成了 5176 个表达序列标签 (EST) 并聚类成 2921 个 unigene。在这些unigenes中，2547个与代表多种推定功能的非冗余蛋白质数据库具有显着的同源性。这些基因的同源物调节不定根的许多方面，包括生长素信号转导和根毛发育的方面。实时定量聚合酶链反应结果显示，AUX1、IRE和FB1在不定根中高表达，且AUX1、ARF1、NAC1、RHD1和IRE的表达在不定根发育过程中增加。此外，从 166 个 EST 中鉴定出 181 个简单序列重复，其中超过 91.16% 是二核苷酸和三核苷酸重复。据我们所知，本研究首次报告了鹅掌楸属中与不定根相关的基因的鉴定，并为未来的基因组研究提供了宝贵的资源。对选定基因的表达分析可以使我们识别对不定根可能至关重要的调控基因。
Liriodendron hybrids (Liriodendron chinense x L. tulipifera) are important landscaping and afforestation hardwood trees. To date, little genomic research on adventitious rooting has been reported in these hybrids, as well as in the genus Liriodendron. In the present study, we used adventitious roots to construct the first cDNA library for Liriodendron hybrids. A total of 5176 expressed sequence tags (ESTs) were generated and clustered into 2921 unigenes. Among these unigenes, 2547 had significant homology to the non-redundant protein database representing a wide variety of putative functions. Homologs of these genes regulated many aspects of adventitious rooting, including those for auxin signal transduction and root hair development. Results of quantitative real-time polymerase chain reaction showed that AUX1, IRE, and FB1 were highly expressed in adventitious roots and the expression of AUX1, ARF1, NAC1, RHD1, and IRE increased during the development of adventitious roots. Additionally, 181 simple sequence repeats were identified from 166 ESTs and more than 91.16% of these were dinucleotide and trinucleotide repeats. To the best of our knowledge, the present study reports the identification of the genes associated with adventitious rooting in the genus Liriodendron for the first time and provides a valuable resource for future genomic studies. Expression analysis of selected genes could allow us to identify regulatory genes that may be essential for adventitious rooting.