Pathogenicity factors and virulence for rainbow trout (Salmo gairdneri) of motile Aeromonas spp. isolated from a river
Pathogenicity factors and virulence for rainbow trout (Salmo gairdneri) of motile Aeromonas spp. isolated from a river
复制标题
运动型气单胞菌对虹鳟鱼（Salmo gairdneri）的致病因素和毒力。
DOI：
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发表时间：
1990
影响因子：
9.4
通讯作者：
G. Naharro
中科院分区：
文献类型：
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作者：
C. Paniagua;O. Rivero;J. Anguita;G. Naharro
从沿着不同地点收集的水样和沉积物中，经过一年的时间分离出97株能动气单胞菌。定期从所有样品中回收菌株，无论分离来源或季节条件如何。通过API 20 NE系统（Analytab Products，Plainview，N.Y.）分类为嗜水气单胞菌（74株）、温和气单胞菌（11株）和豚鼠气单胞菌（12株）。尽管它们的生化模式具有很高的同源性，但它们对鱼类的毒力程度不同，72.02%的A。63%的A.通过肌肉内攻击，Sobria分离物对鱼类具有毒性，但是当使用腹腔内注射时，观察到较低的毒性频率。全是A。经证实，卡那霉素菌株是无毒的。产酪蛋白酶、溶血素和Vero细胞毒素分别为100%、91%和94.59%。A. sobria分离物。没有发现这些活动之间的相关性和鱼的菌株的毒力程度。大多数疏水性菌株似乎集中在A. cathetes，刺萼草A. sobria和无毒A.亚属群体。已知的毒力标记通常与强毒株（吖啶黄阴性和自沉淀阴性和煮沸后沉淀阳性表型）在研究的菌株总数中的代表性较低，与毒力无关。
Ninety-seven motile Aeromonas strains were isolated over a period of a year from samples of water and sediment collected at different sites along a river. Strains were regularly recovered from all samples, regardless of the source of isolation or seasonal conditions. Isolates were biochemically characterized by the API 20NE system (Analytab Products, Plainview, N.Y.) and classified as Aeromonas hydrophila (74 strains), Aeromonas sobria (11 strains), and Aeromonas caviae (12 strains). Despite the high level of homogeneity observed in their biochemical patterns, they displayed different degrees of virulence for fish; 72.02% of A. hydrophila isolates and 63% of A. sobria isolates were virulent for fish by intramuscular challenge, but lower frequencies of virulence were observed when intraperitoneal injections were used. All A. caviae strains proved to be avirulent. Caseinases, hemolysins, and Vero cytotoxins were produced by 100, 91, and 94.59%, respectively, of A. hydrophila strains and with lower frequencies and lower caseinase activities by A. sobria isolates. No correlation was found between these activities and the degree of virulence of the strains for fish. Most hydrophobic strains seem to be concentrated in A. caviae, A. sobria, and avirulent A. hydrophila groups. Known virulence markers commonly associated with virulent strains (acriflavine negative and self-pelleting negative and precipitation after boiling positive phenotypes) had a low representation in the total of strains studied and were not associated with virulence.