The synthesis and secretion of gastric mucus glycoprotein by mucosal cells cultured in the presence of ethanol.
The synthesis and secretion of gastric mucus glycoprotein by mucosal cells cultured in the presence of ethanol.
复制标题
在乙醇存在下培养的粘膜细胞合成和分泌胃粘液糖蛋白。
DOI：
10.1016/0003-9861(85)90670-8
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发表时间：
1985
影响因子：
3.9
通讯作者：
Slomiany,BL
中科院分区：
文献类型：
--
作者：
Slomiany,A;Takagi,A;Slomiany,BL
本文观察了乙醇对胃粘膜细胞粘液糖蛋白合成和分泌的影响。将粘膜细胞悬液在0- 1.5甲醇存在下进行短期（4 h）培养，用[3 H]脯氨酸和[3 H]棕榈酸作为糖蛋白合成和酰化的标记物。从孵育培养基（细胞外糖蛋白）和粘膜细胞（细胞内糖蛋白）中分离合成的标记粘液糖蛋白，并进行分析。根据细胞培养基中的乙醇浓度，观察到对粘液糖蛋白的合成和分泌的两种不同的影响。在0.02- 0.1%甲醇存在下培养的细胞，[~ 3 H]脯氨酸和[~ 3 H]棕榈酸的掺入能力和分泌新组装的粘液糖蛋白的能力均增强。糖蛋白的合成增加了18倍，酰化增加了5倍，分泌增加了10倍。然而，合成的糖蛋白含有的酰基结合脂肪酸少了四到五倍。0.1- 1.5M的乙醇使粘液糖蛋白的合成显著减少（62-64%），但释放到培养基中的糖蛋白量保持不变。这表明，较高浓度的乙醇导致释放预先形成的细胞内粘液糖蛋白储备。结果表明，在乙醇存在下孵育的胃粘膜细胞表现出粘液糖蛋白的合成和分泌受损，并且受损的严重程度取决于乙醇浓度。
The effect of ethanol on the synthesis and secretion of mucus glycoprotein in gastric mucosal cells was investigated. The mucosal cell suspensions were subjected to a short-term (4 h) culture in the presence of 0–1.5methanol, with [3H]proline and [3H]palmitic acid as markers for glycoprotein synthesis and acylation. The synthesized labeled mucus glycoprotein was isolated from the incubation medium (extracellular glycoprotein) and from the mucosal cells (intracellular glycoprotein), and analyzed. Depending upon the ethanol concentration in the cell culture medium, two distinct effects on the synthesis and secretion of mucus glycoprotein were observed. The cells cultured in the presence of 0.02–0.1methanol showed increased ability for the incorporation of [3H]proline and [3H]palmitic acid, and for the secretion of the newly assembled mucus glycoprotein. The synthesis of the glycoprotein increased 18-fold, acylation 5-fold, and secretion 10-fold. The synthesized glycoprotein, however, contained four to five times less of acyl-bound fatty acids. Ethanol at 0.1–1.5mcaused a marked reduction (62–64%) in the mucus glycoprotein synthesis, but the amount of glycoprotein released to the medium remained constant. This indicated that higher concentrations of ethanol caused the release of the preformed intracellular mucus glycoprotein reserves. The results demonstrate that gastric mucosal cells incubated in the presence of ethanol exhibit impaired synthesis and secretion of mucus glycoprotein, and that the severity of impairment depends upon the ethanol concentration.