DIFFERENT GENETIC REQUIREMENTS FOR ANTERIOR RNA LOCALIZATION REVEALED BY THE DISTRIBUTION OF ADDUCIN-LIKE TRANSCRIPTS DURING DROSOPHILA OOGENESIS
DIFFERENT GENETIC REQUIREMENTS FOR ANTERIOR RNA LOCALIZATION REVEALED BY THE DISTRIBUTION OF ADDUCIN-LIKE TRANSCRIPTS DURING DROSOPHILA OOGENESIS
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DOI：
10.1073/pnas.90.6.2512
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发表时间：
1993-03-15
影响因子：
11.1
通讯作者：
LIPSHITZ, HD
中科院分区：
文献类型：
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作者：
DING, D;PARKHURST, SM;LIPSHITZ, HD
由极地定位的mRNAs编码的蛋白质在果蝇胚胎前后轴的细胞命运决定中起着重要的作用。已知的唯一母系合成的mRNA同时定位于卵母细胞和早期胚胎的前皮质，这是双类RNA所必需的，它的定位需要产生一个同源结构域蛋白梯度，该梯度指定沿前后胚轴的位置。我们已经鉴定并鉴定了位于卵母细胞和早期胚胎前极的第二个mRNA。这种信使核糖核酸编码一种哺乳动物内收蛋白的果蝇同源基因，内收蛋白是一种膜-细胞骨架相关蛋白，可以促进光影蛋白-肌动蛋白网络的组装。对母性效应RNA定位突变体exuperantia、slowlow和Staufen中双类和内收蛋白类转录本的空间分布的比较表明，这两个mRNAs正确定位到卵母细胞和早期胚胎的前极的遗传要求不同。
The proteins encoded by polar-localized mRNAs play an important role in cell fate specification along the anteroposterior axis of the Drosophila embryo. The only maternally synthesized mRNA known previously to be localized to the anterior cortex of both the oocyte and the early embryo is the bicoid mRNA whose localization is required to generate a homeodomain protein gradient that specifies position along the anteroposterior embryonic axis. We have identified and characterized a second mRNA that is localized to the anterior pole of the oocyte and early embryo. This mRNA encodes a Drosophila homolog of mammalian adducin, a membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. A comparison of the spatial distribution of bicoid and Adducin-like transcripts in the maternal-effect RNA-localization mutants exuperantia, swallow, and staufen indicates different genetic requirements for proper localization of these two mRNAs to the anterior pole of the oocyte and early embryo.