Analysis of Low-temperature Tolerance of a Tomato (Lycopersicon esculentum) Cybrid with Chloroplasts from a more Chilling-tolerant L. hirsutum Accession
Analysis of Low-temperature Tolerance of a Tomato (Lycopersicon esculentum) Cybrid with Chloroplasts from a more Chilling-tolerant L. hirsutum Accession
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番茄 (Lycopersicon esculentum) 杂种与来自更耐冷的陆地植物种质的叶绿体的低温耐受性分析
DOI：
10.1006/anbo.2000.1142
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
P. Hasselt
中科院分区：
文献类型：
--
作者：
J. Venema;M. Eekhof;P. Hasselt
含有L. L.冷敏感细胞质白化病突变体核基因组的异质番茄（胞质杂种）（即AH 47系）的生长和光合作用。番茄“大红樱桃”（LRC）和一个更耐冷的高海拔加入相关的野生物种L。毛藓& Bonpl. LA 1777在最适（25/208 ℃）和次适（16/148 ℃）昼夜温度制度下进行了研究，并将它们的表现与两个真胞质亲本进行了比较。胞质杂种的生物量和发育速率与核番茄相似。esculentum）亲本在两个温度制度。与适温下生长的地上部生物量相比，亚适温下生长的地上部生物量减少幅度较小。hirsutum比L. esculentum和cybrid。这种差异与其对叶面积扩大的抑制作用强有关。在次适温条件下，番茄和胞质杂种的生长速度比L.多毛植物无论植物生长的温度制度下，光合性能和叶色素，碳水化合物和可溶性蛋白质含量的胞质杂种类似的核父母。没有外星人L的优势。hirsutum叶绿体的生长和光合相关特性的观察在胞质杂种在次适温制度，表明光合机构的温度敏感性是由核基因调控。种间叶绿体转移的一个不利后果是增加的敏感性冷诱导的光抑制的胞质杂种。因此，细胞质杂交不是提高番茄耐低温性的有效手段。#2000 Annals of Botany公司
Growth and photosynthesis of an alloplasmic tomato (cybrid), i.e. line AH47, containing the nuclear genome of the chilling-sensitive cytoplasmic albino mutant of L. esculentum Mill. ‘Large Red Cherry’ (LRC) and the plastome of a more chilling-tolerant high-altitude accession of the related wild species L. hirsutum Humb. & Bonpl. LA 1777, were investigated at an optimal (25/208C) and suboptimal (16/148C) day/night temperature regime and their performance compared with that of both euplasmic parents. The cybrid shoot had a similar biomass and development rate to the nuclear tomato (L. esculentum) parent at both temperature regimes. Compared with the biomass production of shoots grown at optimal temperature, the reduction in shoot biomass at suboptimal temperature was smaller for L. hirsutum than for L. esculentum and the cybrid. This diAerence was related to a stronger inhibition of leaf area expansion in L. esculentum and the cybrid in the suboptimal temperature regime than in L. hirsutum. Irrespective of the temperature regime under which the plants were grown, photosynthetic performance and leaf pigment, carbohydrate and soluble-protein contents of the cybrid resembled those of the nuclear parent. No advantages of the alien L. hirsutum chloroplast with respect to growth and photosynthesis-related characteristics were observed in the cybrid in the suboptimal temperature regime, indicating that the temperature sensitivity of the photosynthetic apparatus is regulated by nuclear genes. An adverse consequence of interspecific chloroplast transfer was the increased susceptibility to chill-induced photoinhibition of the cybrid. It is concluded that cybridization is not a useful tool for improving low-temperature tolerance of tomato. # 2000 Annals of Botany Company