Association between plasma CC16 levels, the A38G polymorphism, and asthma
Association between plasma CC16 levels, the A38G polymorphism, and asthma
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DOI：
10.1164/ajrccm.161.1.9904073
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发表时间：
2000-01-01
影响因子：
24.7
通讯作者：
Le Souëf, PN
中科院分区：
文献类型：
--
作者：
Laing, IA;Hermans, C;Le Souëf, PN
通过检测100例哮喘和非哮喘儿童血浆CC16水平，探讨A38G基因多态性对Clara细胞分泌蛋白(CC16)基因表达及哮喘发病的影响。采用乳胶免疫法测定A38G基因分型和血浆CC16水平。经年龄和性别调整后，哮喘组平均血浆CC16水平(7.96mU/L；95%可信区间[CI]=6.79~9.31)低于非哮喘组(9.98mU/L；95%CI=8.83~11.26)(P=0.006)。同样，38A/38A受试者的平均血浆CC16水平(6.79ug/L；95%CI=4.56~9.02)也低于38G/38G受试者(10.01ug/L；95%CI=7.9~12.12；p=0.003)。与38G/38G组相比，38A/38A组诊断哮喘的优势比为4.78(95%CI=1.08~21.18；P=0.04)。然而，当从血浆CC16水平校正后，这一点降低了，这表明哮喘的几率在很大程度上是通过改变血浆CC16水平来调节的。38A序列与血浆CC16水平降低相关，血浆CC16水平较低的个体更有可能患有哮喘。这进一步证明了CC16基因38A等位基因在哮喘发病中的重要作用。
The effect of the A38G polymorphism on Clara cell secretary protein (CC16) gene expression and asthma was investigated by measuring plasma CC16 levels in 100 asthmatic and nonasthmatic children. Restriction digestion determined the A38G genotype and plasma CC16 levels were analyzed using a sensitive latex immunoassay. Asthmatics had lower mean plasma CC16 levels adjusted for age and gender (7.96 mu g/L; 95% confidence interval [Cl] = 6.79 to 9.31) than nonasthmatic subjects (9.98 mu g/L; 95% CI = 8.83 to 11.26) (p = 0.006). Similarly adjusted, mean plasma CC16 levels were also lower in 38A/38A (6.79 mu g/L; 95% CI = 4.56 to 9.02) than 38G/38G subjects (10.01 mu g/L; 95% CI = 7.90 to 12.12; p = 0.003). The odds ratio for asthma diagnosis of 38A/38A subjects was 4.78 (95% CI = 1.08 to 21.18; p = 0.04) compared with 38G/38G subjects. However, this was reduced when corrected from plasma CC16 level suggesting that the odds of asthma was largely mediated through altered plasma CC16 levels. The 38A sequence was associated with reduced plasma CC16 levels and individuals with lower plasma CC16 levels were more likely to have asthma. This provides further evidence far a significant role of the CC16 gene, 38A allele in the development of asthma.