Sequence Type ST131 and ST10 Complex (ST617) predominant among CTX-M-15-producing Escherichia coli isolates from Nigeria
Sequence Type ST131 and ST10 Complex (ST617) predominant among CTX-M-15-producing Escherichia coli isolates from Nigeria
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DOI：
10.1111/j.1469-0691.2011.03730.x
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发表时间：
2012-03-01
影响因子：
14.2
通讯作者：
Ghebremedhin, B.
中科院分区：
文献类型：
--
作者：
Aibinu, I.;Odugbemi, T.;Ghebremedhin, B.
从拉各斯的两个主要三级医院（大学教学医院和国家骨科医院Igbobi）分离的109株临床大肠埃希菌中，14株（12.8%）产超广谱β-内酰胺酶（ESBL）菌株采用PCR和测序、ERIC-PCR和多位点序列分型进行了表征。所有产ESBL菌株仅编码CTX-M-15基因。克隆群ST 131（35.7%）是最主要的ST，其次是ST 617（28.6%）。还观察到其他序列类型的孤立病例。质粒介导的喹诺酮类耐药基因qnrA、qnrB 1和aac-（6 '）-lb-cr在不同克隆群的ESBL菌株中均有检出。这是第一次描述产CTX-M-15的E.产自尼日利亚。不同克隆谱系的存在表明了遗传多样化和新流行毒株出现的持续潜力。
Of 109 clinical Escherichia coli isolates from two major tertiary hospitals in Lagos (University Teaching Hospital and the National Orthopaedic Hospital Igbobi), 14 (12.8%) extended-spectrum beta-lactamse (ESBL) producers were characterized using PCR and sequencing, ERIC-PCR and multilocus sequence typing. All ESBL-producing isolates encoded only the CTX-M-15 gene. Clonal group ST131 (35.7%) was the predominant ST, followed by ST617 (28.6%). Isolated cases of other sequence types were also observed. Plasmid-mediated quinolone resistance genes qnrA, qnrB1 and aac-(6')-lb-cr were detected among these ESBL isolates of different clonal groups. This is the first description of the clonality of CTX-M-15-producing E. coli from Nigeria. The presence of diverse clonal lineages shows the continuing potential for genetic diversification and emergence of new epidemic strains.